Document Detail

Quantitative detection of secretory immunoglobulin A to Helicobacter pylori in gastric juice: antibody capture enzyme-linked immunosorbent assay.
MedLine Citation:
PMID:  8852358     Owner:  NLM     Status:  MEDLINE    
Helicobacter pylori is a major etiologic agent in gastroduodenal disorders. In this study, immunoglobulin A (IgA) antibodies to H. pylori were estimated in serum and gastric juice specimens from patients with gastritis and peptic ulcers using antibody capture enzyme-linked immunosorbent assays (ACELISAs). The antibody titers of the ACELISAs are independent of the antibody concentration and reflect the ratio of H. pylori-specific IgA to total IgA. The ratio is stable, although the antibody concentration fluctuates in gastric juice. Using the ACELISAs it was possible to evaluate quantitatively not only serum IgA (SR-IgA) antibodies but also secretory IgA (SC-IgA) antibodies in gastric juice. There were significant differences between the patients and control group in the SR-IgA and SC-IgA ACELISAs. Furthermore, the ACELISAs made it possible to compare between SR-IgA antibodies in serum and SC-IgA antibodies in gastric juice. In all patients, the ratios of H. pylori-specific IgA were higher in gastric juice than in serum. These results suggest that H. pylori SC-IgA antibodies are mainly produced by the local immune response in the gastric mucosa. Our studies indicate that ACELISA is well suited for the analysis of local immune response in mucosa.
S Hayashi; T Sugiyama; K Hisano; T Awakawa; I Kurokawa; A Yachi; H Isogai; E Isogai; K Yokota; Y Hirai; K Oguma; N Fujii
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of clinical laboratory analysis     Volume:  10     ISSN:  0887-8013     ISO Abbreviation:  J. Clin. Lab. Anal.     Publication Date:  1996  
Date Detail:
Created Date:  1996-12-06     Completed Date:  1996-12-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8801384     Medline TA:  J Clin Lab Anal     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  74-7     Citation Subset:  IM    
Department of Microbiology, Sapporo Medical University, Japan.
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MeSH Terms
Aged, 80 and over
Enzyme-Linked Immunosorbent Assay / methods
Gastric Juice / immunology*,  microbiology
Gastric Mucosa / immunology,  microbiology
Helicobacter Infections / diagnosis,  immunology*
Helicobacter pylori / immunology*,  isolation & purification
Immunoglobulin A / blood
Immunoglobulin A, Secretory / immunology,  isolation & purification*
Middle Aged
Reg. No./Substance:
0/Immunoglobulin A; 0/Immunoglobulin A, Secretory

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