Document Detail


Quantitative detection method for Roundup Ready soybean in food using duplex real-time PCR MGB chemistry.
MedLine Citation:
PMID:  20549794     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Methodologies that enable the detection of genetically modified organisms (GMOs) (authorized and non-authorized) in food and feed strongly influence the potential for adequate updating and implementation of legislation together with labeling requirements. Quantitative polymerase chain reaction (qPCR) systems were designed to boost the sensitivity and specificity on the identification of GMOs in highly degraded DNA samples; however, such testing will become economically difficult to cope with due to increasing numbers of approved genetically modified (GM) lines. Multiplexing approaches are therefore in development to provide cost-efficient solution. RESULTS: Construct-specific primers and probe were developed for quantitative analysis of Roundup Ready soybean (RRS) event glyphosate-tolerant soybean (GTS) 40-3-2. The lectin gene (Le1) was used as a reference gene, and its specificity was verified. RRS- and Le1-specific quantitative real-time PCR (qRTPCR) were optimized in a duplex platform that has been validated with respect to limit of detection (LOD) and limit of quantification (LOQ), as well as accuracy. The analysis of model processed food samples showed that the degradation of DNA has no adverse or little effects on the performance of quantification assay. CONCLUSION: In this study, a duplex qRTPCR using TaqMan minor groove binder-non-fluorescent quencher (MGB-NFQ) chemistry was developed for specific detection and quantification of RRS event GTS 40-3-2 that can be used for practical monitoring in processed food products.
Authors:
Maria Cristina Samson; Mariolina Gullì; Nelson Marmiroli
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  Journal of the science of food and agriculture     Volume:  90     ISSN:  1097-0010     ISO Abbreviation:  J. Sci. Food Agric.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-15     Completed Date:  2010-10-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376334     Medline TA:  J Sci Food Agric     Country:  England    
Other Details:
Languages:  eng     Pagination:  1437-44     Citation Subset:  IM    
Affiliation:
Department of Environmental Sciences, Genetics and Environmental Biotechnology Section, University of Parma, Parma, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animal Feed / analysis
Bread / analysis
DNA, Plant / analysis
European Union
Fluorescent Dyes
Food Analysis / methods*
Food Inspection / economics,  methods
Food Labeling / legislation & jurisprudence
Food, Genetically Modified / adverse effects
Glycine / analogs & derivatives
Herbicide Resistance / genetics*
Herbicides
Intercalating Agents / chemistry
Limit of Detection
Plants, Genetically Modified / genetics*
Polymerase Chain Reaction / methods*
Reproducibility of Results
Soybeans / classification,  genetics*
Chemical
Reg. No./Substance:
0/DNA, Plant; 0/Fluorescent Dyes; 0/Herbicides; 0/Intercalating Agents; 1071-83-6/glyphosate; 56-40-6/Glycine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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