| Quantitative cytochemical detection of malignant and potentially malignant cells in the colon. | |
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MedLine Citation:
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PMID: 2379174 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It was found to be possible to distinguish malignant cells from normal cells by using an oxygen-sensitive tetrazolium salt (neotetrazolium) for the histochemical demonstration of glucose-6-phosphate dehydrogenase activity in cryostat sections of human colon. We have studied 12 cases of established adenocarcinoma of the colon in addition to 4 of ulcerative colitis and 4 of adenomatous polyposis (polyposis coli). In a nitrogen atmosphere the activities of malignant and normal cells were similar. However, after incubation in an atmosphere of pure oxygen, only malignant cells gave a positive reaction after 5 min. Three of the four cases of adenomatous polyposis gave a positive reaction for glucose-6-phosphate dehydrogenase activity in oxygen in a manner similar to that of specimens with severe dysplasia. In general, positive foci were histologically indistinguishable from the neighboring tubuli. However, foci of severely dysplastic epithelium usually showed a positive reaction. All three patients eventually developed clear-cut severe dysplasia. The other patient, who showed a negative reaction in oxygen, was diagnosed after 3 years as not suffering from dysplasia. All cases of ulcerative colitis gave a reaction in oxygen comparable with that of normal cells. Therefore, the areas with a positive reaction are considered to be either in the process of malignant transformation or malignant. An explanation for the oxygen insensitivity of cancer cells appeared to be a decrease in the activity of superoxide dismutase (EC 1.15.1.1), as addition of exogenous superoxide dismutase to malignant cells caused a normal reaction. We wish to suggest that this test in combination with the routine histology may be exploited for the diagnosis of polyps in premalignant conditions. |
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Authors:
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A J Best; P K Das; H R Patel; C J Van Noorden |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cancer research Volume: 50 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 1990 Aug |
Date Detail:
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Created Date: 1990-09-12 Completed Date: 1990-09-12 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 5112-8 Citation Subset: IM |
Affiliation:
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Laboratory of Cell Biology and Histology, University of Amsterdam, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
/
enzymology,
pathology* Aged Aged, 80 and over Colitis, Ulcerative / enzymology, pathology* Colon / cytology, enzymology, pathology* Colonic Neoplasms / enzymology, pathology* Colonic Polyps / enzymology, pathology Epithelium / enzymology, pathology Female Glucosephosphate Dehydrogenase / analysis*, metabolism Humans Kinetics Male Middle Aged Muscle, Smooth / cytology, enzymology, pathology Tumor Markers, Biological / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Tumor Markers, Biological; EC 1.1.1.49/Glucosephosphate Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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