| Quantitative comparison of in situ methods for detecting apoptosis induced by X-ray irradiation in mouse thymus. | |
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MedLine Citation:
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PMID: 9466149 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Apoptosis, or programmed cell death, is a process fundamental to the homeostasis of multicellular organisms. Therefore, the development of methods for detecting dying and dead cells is of great importance. In the present study, four methodologies for identifying thymocyte apoptosis were evaluated and compared: a classical Haematoxylin and Eosin staining method, two histochemical methods (DNA polymerase-mediated in situ end-labelling and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end-labelling) and flow cytometry. Aspects important for the quantitation of apoptosis in the mouse thymus after a low dose (below 1 Gy) of X-ray irradiation were emphasized. The nick-end-labelling method had the highest sensitivity among the four methods in detecting apoptotic cells; however, the in situ end-labelling method showed sensitivity similar to nick-end-labelling and possessed better response to incremental increase in radiation. The sensitivity of the Haematoxylin and Eosin staining was lower than the above two methods. Flow cytometry could not detect low-frequency apoptosis after a low radiation dose of below 30 cGy (cGy = 0.01 Gy) and did not respond linearly to increasing radiation. We conclude, therefore, that the in situ end-labelling method is the most adequate of the methods tested, especially for the detection of low-frequency apoptosis. |
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Authors:
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K Fujita; H Ohyama; T Yamada |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: The Histochemical journal Volume: 29 ISSN: 0018-2214 ISO Abbreviation: Histochem. J. Publication Date: 1997 Nov-Dec |
Date Detail:
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Created Date: 1998-03-03 Completed Date: 1998-03-03 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0163161 Medline TA: Histochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 823-30 Citation Subset: IM |
Affiliation:
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Department of Biology, Toho University School of Medicine, Tokyo, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis* DNA Fragmentation* DNA Nucleotidylexotransferase / metabolism DNA-Directed DNA Polymerase / metabolism Evaluation Studies as Topic Female Flow Cytometry Histocytochemistry / methods Mice Mice, Inbred AKR Sensitivity and Specificity Staining and Labeling Thymus Gland / cytology*, radiation effects* |
| Chemical | |
Reg. No./Substance:
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EC 2.7.7.31/DNA Nucleotidylexotransferase; EC 2.7.7.7/DNA-Directed DNA Polymerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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