Document Detail


Quantitative comparison of casein and rapeseed proteolysis by pancreatin.
MedLine Citation:
PMID:  8921618     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The problem of quantitative comparison of kinetic curves was solved for casein and rapeseed pancreatin hydrolysis in a membrane reactor, which ensured the measurement of proteolysis kinetics for the products with a molecular weight of less than 1000. Coordinates were derived which provided good linearization of kinetic curves and the determination of relative rate constants irrespective of reagent concentrations, E0/S0 ratio and time intervals of kinetic measurements. When the relative rate constants of the release of the individual amino acid residues in the low-weight proteolysis products were compared, trypsin-dependent constants (for Lys and Arg residues) were found to be two times less for rapeseed than for casein, and chymotrypsin-dependent constants (for Tyr and Phe residues) were approximately 1.3 times higher for rapeseed than for casein. Statistical analysis demonstrated that the distribution of constants was narrower for rapeseed than for casein. Differences between target (Arg, Lys, Tyr and Phe) and non-target constants of release in the form of peptides and free amino acids, or in the form of free amino acids only, were attributed on the differences in the peptide bond masking for casein and rapeseed proteins. Computer simulation of proteolysis kinetics was performed by PROTEOLYSIS program package to confirm the dependence of rate constant distribution on the state of masking.
Authors:
M M Vorob'ev; G Parent; L Savoie
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Die Nahrung     Volume:  40     ISSN:  0027-769X     ISO Abbreviation:  Nahrung     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1997-01-03     Completed Date:  1997-01-03     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0142530     Medline TA:  Nahrung     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  248-55     Citation Subset:  IM    
Affiliation:
Foodinform Ltd., Moscow, Russia.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Brassica / chemistry*
Caseins / analysis,  chemistry*
Computer Simulation
Hydrolysis
Kinetics
Models, Chemical
Molecular Weight
Pancreatin / chemistry*
Plant Proteins / analysis,  chemistry*
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Caseins; 0/Plant Proteins; 8049-47-6/Pancreatin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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