| Quantitative body composition analysis in awake mice and rats by magnetic resonance relaxometry. | |
| | |
MedLine Citation:
|
PMID: 15536224 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: Magnetic resonance (MR) relaxometry has recently been introduced for noninvasive body composition analysis in awake mice. The purpose of the present study was to extend the method to rats and to introduce calibration procedures that render MR relaxometry fully quantitative. RESEARCH METHODS AND PROCEDURES: Proton T(2) MR relaxometry at 4.7 Tesla was used for body composition analyses in 700 awake mice and 400 rats of different strains and conditions. Relaxograms calculated from the signal decays observed with multi-spin-echo acquisition provided well-separated contributions of tissue water and fat. Analysis of fat composition was carried out in vivo using (13)C-MR spectroscopy. Evolution of body composition in rats was assessed during drug treatment. RESULTS: MR relaxometry for noninvasive body composition analysis in laboratory rodents was implemented on a standard MR scanner, and a throughput of >30 animals per hour was achieved. Excellent linearity and reproducibility with coefficients of variance as low as 2.5% and 1.7% were obtained in mice and rats, respectively. The lean mass-to-water ratio (mice, 1.35 +/- 0.03; rats, 1.39 +/- 0.04) and the proton density of fat (mice, 8.1 +/- 0.2; rats, 8.9 +/- 0.2 g/mol) were determined from cross-sectional data. Fat composition analysis by (13)C-MR spectroscopy corroborated these findings and yielded information on the average acyl chain length (16.3 +/- 1.6) and contributions of saturated (27 +/- 3%), monounsaturated (22 +/- 2%), and polyunsaturated (51 +/- 3%) fatty acids. Longitudinal assessments in rats treated with sibutramine and dexfenfluramine showed dose-related changes in body composition. DISCUSSION: T(2) MR relaxometry backed by solid calibration provides a powerful means for rapid quantitative body composition analysis in awake mice and rats that is suitable for serial investigations in pharmaceutical research. |
| | |
Authors:
|
Basil Künnecke; Philippe Verry; Agnès Bénardeau; Markus von Kienlin |
Related Documents
:
|
9530224 - Thermoeffector thresholds and preferred ambient temperatures of the fok rat. 10494994 - Acute toxicity of 3,4-methylenedioxymethamphetamine (mdma) in sprague-dawley and dark a... 233774 - Tissue metabolism and enzyme activities in the rodent heterocephalus glaber, a poor tem... 7478194 - Long-term potentiation at low temperature is stronger in hippocampal slices from hibern... 12751804 - Auditory event-related potentials in humans and rats: effects of task manipulation. 826174 - Unidirectional uptake of iron across intestinal brush border. |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: Obesity research Volume: 12 ISSN: 1071-7323 ISO Abbreviation: Obes. Res. Publication Date: 2004 Oct |
Date Detail:
|
Created Date: 2004-11-10 Completed Date: 2005-04-08 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9305691 Medline TA: Obes Res Country: United States |
Other Details:
|
Languages: eng Pagination: 1604-15 Citation Subset: IM |
Affiliation:
|
Magnetic Resonance Imaging and Spectroscopy, PRBD-M, Building 68/05A, F. Hoffmann-La Roche Ltd., Grenzacherstrasse 124, CH-4070 Basel, Switzerland. basil.kuennecke@roche.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Anti-Obesity Agents / therapeutic use* Body Composition* Calibration Cross-Sectional Studies Cyclobutanes Dexfenfluramine Magnetic Resonance Spectroscopy / methods, standards* Mice Mice, Inbred AKR Mice, Inbred C57BL Mice, Obese Obesity / diet therapy, drug therapy* Rats Rats, Sprague-Dawley Rats, Wistar Rats, Zucker Reproducibility of Results Sensitivity and Specificity Weight Loss / physiology |
| Chemical | |
Reg. No./Substance:
|
0/Anti-Obesity Agents; 0/Cyclobutanes; 106650-56-0/sibutramine; 3239-44-9/Dexfenfluramine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: S 23521 decreases food intake and body weight gain in diet-induced obese rats.
Next Document: Adapted changes in left ventricular structure and function in severe uncomplicated obesity.