| Quantitative blood flow measurements in the small animal cardiopulmonary system using digital subtraction angiography. | |
| | |
MedLine Citation:
|
PMID: 19994543 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
PURPOSE: The use of preclinical rodent models of disease continues to grow because these models help elucidate pathogenic mechanisms and provide robust test beds for drug development. Among the major anatomic and physiologic indicators of disease progression and genetic or drug modification of responses are measurements of blood vessel caliber and flow. Moreover, cardiopulmonary blood flow is a critical indicator of gas exchange. Current methods of measuring cardiopulmonary blood flow suffer from some or all of the following limitations--they produce relative values, are limited to global measurements, do not provide vasculature visualization, are not able to measure acute changes, are invasive, or require euthanasia. METHODS: In this study, high-spatial and high-temporal resolution x-ray digital subtraction angiography (DSA) was used to obtain vasculature visualization, quantitative blood flow in absolute metrics (ml/min instead of arbitrary units or velocity), and relative blood volume dynamics from discrete regions of interest on a pixel-by-pixel basis (100 x 100 microm2). RESULTS: A series of calibrations linked the DSA flow measurements to standard physiological measurement using thermodilution and Fick's method for cardiac output (CO), which in eight anesthetized Fischer-344 rats was found to be 37.0 +/- 5.1 ml/min. Phantom experiments were conducted to calibrate the radiographic density to vessel thickness, allowing a link of DSA cardiac output measurements to cardiopulmonary blood flow measurements in discrete regions of interest. The scaling factor linking relative DSA cardiac output measurements to the Fick's absolute measurements was found to be 18.90 x CODSA = COFick. CONCLUSIONS: This calibrated DSA approach allows repeated simultaneous visualization of vasculature and measurement of blood flow dynamics on a regional level in the living rat. |
| | |
Authors:
|
MingDe Lin; Craig T Marshall; Yi Qi; Samuel M Johnston; Cristian T Badea; Claude A Piantadosi; G Allan Johnson |
Related Documents
:
|
130573 - Metabolic clearance rate of dehydroisoandrosterone sulfate. vii. effect of lateral vers... 8335533 - Uteroplacental blood flow at rest and during exercise in late-gestation conscious rats. 8900293 - Exposure of blood to biomaterial surfaces liberates substances that activate polymorpho... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
|
Title: Medical physics Volume: 36 ISSN: 0094-2405 ISO Abbreviation: Med Phys Publication Date: 2009 Nov |
Date Detail:
|
Created Date: 2009-12-09 Completed Date: 2010-01-12 Revised Date: 2011-08-01 |
Medline Journal Info:
|
Nlm Unique ID: 0425746 Medline TA: Med Phys Country: United States |
Other Details:
|
Languages: eng Pagination: 5347-58 Citation Subset: IM |
Affiliation:
|
Department of Radiology, Center for In Vivo Microscopy, Duke University Medical Center, Box 3302, Durham, North Carolina 27710, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Angiography, Digital Subtraction
/
methods* Animals Calibration Heart / anatomy & histology, physiology* Lung / anatomy & histology, physiology* Male Phantoms, Imaging Rats Rats, Inbred F344 Regional Blood Flow* Thermodilution |
| Grant Support | |
ID/Acronym/Agency:
|
P41 RR005959/RR/NCRR NIH HHS; P41 RR005959-20/RR/NCRR NIH HHS; U24 CA092656/CA/NCI NIH HHS; U24 CA092656-09/CA/NCI NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Initial investigation on the use of MR spectroscopy and micro-MRI of GAFCHROMIC EBT radiotherapy fil...
Next Document: IMRT commissioning: multiple institution planning and dosimetry comparisons, a report from AAPM Task...