| Quantitative assessment of toxic and nontoxic Microcystis colonies in natural environments using fluorescence in situ hybridization and flow cytometry. | |
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MedLine Citation:
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PMID: 20821296 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Toxic cyanobacterial blooms constitute a threat to human safety because Microcystis sp. releases microcystins during growth, and particularly during cell death. Therefore, analysis of toxic and nontoxic Microcystis in natural communities is required in order to assess and predict bloom dynamics and toxin production by these organisms. In this study, an analysis combining fluorescence in situ hybridization (FISH) with flow cytometry (FCM) was used to discriminate between toxic and nontoxic Microcystis and also to quantify the percentage of toxic Microcystis present in blooms. The results demonstrate that the combination of FISH and flow cytometry is a useful approach for studying the ecology of Microcystis toxin production and for providing an early warning for toxic Microcystis blooms. |
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Authors:
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NanQin Gan; Qun Huang; LingLing Zheng; LiRong Song |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-07 |
Journal Detail:
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Title: Science China. Life sciences Volume: 53 ISSN: 1869-1889 ISO Abbreviation: Sci China Life Sci Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-09-07 Completed Date: 2010-12-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101529880 Medline TA: Sci China Life Sci Country: China |
Other Details:
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Languages: eng Pagination: 973-80 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence DNA Primers Flow Cytometry In Situ Hybridization, Fluorescence Microcystis / isolation & purification* Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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