| Quantitative assessment of the relative contributions of steric repulsion and chemical interactions to macromolecular crowding. | |
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MedLine Citation:
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PMID: 23348671 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The term "macromolecular crowding" denotes the combined effects of high volume fractions of nominally unrelated macromolecules upon the equilibrium and transport properties of all macrosolutes, dilute as well as concentrated, in the crowded medium. We present a formal partitioning of the total crowding effect into contributions from steric exclusion (excluded volume) and weak, nonspecific attractive interactions between a concentrated "crowding agent" and reactant and product species present at trace concentration. A numerical example of the combined effect of both steric and chemical interactions between crowder and tracer upon the reversible dimerization of tracer is presented, based upon reasonable estimates of the magnitude of both repulsive and attractive interactions between tracer and crowder species. © 2012Wiley Periodicals, Inc. Biopolymers 99: 239-244, 2013. |
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Authors:
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Allen P Minton |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biopolymers Volume: 99 ISSN: 0006-3525 ISO Abbreviation: Biopolymers Publication Date: 2013 Apr |
Date Detail:
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Created Date: 2013-01-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372525 Medline TA: Biopolymers Country: United States |
Other Details:
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Languages: eng Pagination: 239-44 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
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Section on Physical Biochemistry, Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, U.S. Department of Health and Human Services, Bethesda, MD. minton@helix.nih.gov. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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