| Quantitative assessment of macrophage functions in repair and fibrosis. | |
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MedLine Citation:
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PMID: 21462164 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Macrophages play key roles in wound repair and fibrosis by regulating extracellular matrix turnover. Macrophages can process matrix components themselves, but also recruit and alter the functions of other cell types that directly build or degrade extracellular matrix. Classically activated macrophages (CAM, also called M1) tend to promote tissue injury while alternatively activated macrophages (AAM, also called M2) are often linked with the mechanisms of wound repair and fibrosis. However, rather than promoting collagen deposition, recent studies suggest that arginase-1-expressing AAM suppress chronic inflammation and fibrosis by inhibiting antigen-specific T cell responses. This unit describes methods to measure arginase activity in macrophages and whole tissues as well as assays to quantify the T cell suppressive activity of AAMs. Modified hydroxyproline and soluble collagen assays that can be used to quantify collagen levels in tissues and brochoalveolar lavage fluid are also described. The protocols in this unit should provide the investigator with all the necessary information required to measure arginase activity and to correlate the observed activity with the progression and resolution of fibrosis. |
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Authors:
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Thomas A Wynn; Luke Barron; Robert W Thompson; Satish K Madala; Mark S Wilson; Allen W Cheever; Thirumalai Ramalingam |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Current protocols in immunology / edited by John E. Coligan ... [et al.] Volume: Chapter 14 ISSN: 1934-368X ISO Abbreviation: Curr Protoc Immunol Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-04-04 Completed Date: 2011-08-23 Revised Date: 2012-04-04 |
Medline Journal Info:
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Nlm Unique ID: 9101651 Medline TA: Curr Protoc Immunol Country: United States |
Other Details:
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Languages: eng Pagination: Unit14.22 Citation Subset: IM |
Copyright Information:
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© 2011 by John Wiley & Sons, Inc. |
Affiliation:
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National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arginase / metabolism Fibrosis Humans Immunologic Techniques* Macrophage Activation / immunology* Macrophages / immunology*, metabolism Mice T-Lymphocytes / immunology Wound Healing* |
| Grant Support | |
ID/Acronym/Agency:
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ZIA AI000829-13/AI/NIAID NIH HHS; ZIA AI001019-04/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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EC 3.5.3.1/Arginase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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