| Quantitative assessment of early experimental diabetes in rats using dynamic contrast-enhanced computed tomography. | |
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MedLine Citation:
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PMID: 19346093 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To quantitatively assess the time course of changes of the renal volume and function in the early phase of streptozotocin (STZ)-induced diabetes in rats using dynamic contrast-enhanced computed tomography (DCE-CT). METHODS: The DCE-CT studies were performed in 24 male Sprague-Dawley rats (n=6 for control and n=18 for STZ-treated group) on days 0, 4, 7, 11, and 14 using a multi-detector row CT. The rats of an STZ-treated group were given intraperitoneally 65mg/kg body weight of STZ on day 0, and were divided into two groups based on the blood glucose concentration on day 4 being less than 300mg/dL [STZ-treated group (L), n=8] or greater than 300mg/dL [STZ-treated group (G), n=10]. The contrast clearance per unit renal volume (K(1)) was estimated from the DCE-CT data using the Patlak model. The renal volume (V(CT)) was calculated by manually delineating the kidney on the contrast-enhanced CT image. The contrast clearance of the entire kidney (K) was obtained by K(1)xV(CT). RESULTS: V(CT) in the STZ-treated group was significantly enlarged on day 4 compared to that on day 0 and continued until day 14. Although there were no significant changes in the time course of K(1) in all groups, K in the STZ-treated groups (L) and (G) significantly increased on days 7 and 4, respectively, and continued until day 14, suggesting that hyperfiltration occurs in parallel with renal volume enlargement. CONCLUSION: The present method appears useful for quantitatively evaluating the time course of STZ-induced diabetes in rats, because it allows repeated and simultaneous evaluation of renal morphology and function. |
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Authors:
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Kenya Murase; Akihiro Kitamura; Atsushi Tachibana; Yoshinori Kusakabe; Risa Matsuura; Shohei Miyazaki |
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Publication Detail:
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Type: Journal Article Date: 2009-04-05 |
Journal Detail:
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Title: European journal of radiology Volume: 74 ISSN: 1872-7727 ISO Abbreviation: Eur J Radiol Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-04-05 Completed Date: 2010-08-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8106411 Medline TA: Eur J Radiol Country: Ireland |
Other Details:
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Languages: eng Pagination: 280-6 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
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Department of Medical Physics and Engineering, Division of Medical Technology and Science, Faculty of Health Science, Graduate School of Medicine, Osaka University, 1-7 Yamadaoka, Suita, Osaka 565-0871, Japan. murase@sahs.med.osaka-u.ac.jp |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Contrast Media Diabetes Mellitus, Experimental Diabetes Mellitus, Type 2 / diagnosis*, physiopathology Kidney / pathology Male Rats Rats, Sprague-Dawley Renal Insufficiency Time Factors Tomography, X-Ray Computed* |
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Reg. No./Substance:
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0/Contrast Media |
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