Document Detail

Quantitative analysis of yeast internal architecture using soft X-ray tomography.
MedLine Citation:
PMID:  21181783     Owner:  NLM     Status:  Publisher    
We used soft X-ray tomography (SXT)-a high-resolution, quantitative imaging technique-to measure cell size and organelle volumes in yeasts. Cell size is a key factor in initiating cell division in yeasts, whereas the number and volume of the organelles have a profound impact on the function and viability of a cell. Consequently, determining these cell parameters is fundamentally important in understanding yeast biology. SXT is well suited to this type of analysis. Specimens are imaged in a near-native state, and relatively large numbers of cells can be readily analysed. In this study, we characterized haploid and diploid strains of Saccharomyces cerevisiae at each of the key stages in the cell cycle and determined the relationships that exist cellular and organelle volumes. We then compared these results with SXT data obtained from Schizosaccharomyces pombe, the three main phenotypes displayed by the opportunistic yeast pathogen Candida albicans and from a coff1-22 mutant strain of S. cerevisiae. This comparison revealed that volumetric ratios were invariant, irrespective of yeast strain, ploidy or morphology, leading to the conclusion these volumetric ratios are common in all yeasts. Copyright © 2010 John Wiley & Sons, Ltd.
Maho Uchida; Yidi Sun; Gerry McDermott; Christian Knoechel; Mark A Le Gros; Dilworth Parkinson; David G Drubin; Carolyn A Larabell
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2010-11-15
Journal Detail:
Title:  Yeast (Chichester, England)     Volume:  -     ISSN:  1097-0061     ISO Abbreviation:  -     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-12-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8607637     Medline TA:  Yeast     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Anatomy, University of California at San Francisco, CA, USA.
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