Document Detail


Quantitative analysis of viral burden in tissues from adults and children with symptomatic human immunodeficiency virus type 1 infection assessed by polymerase chain reaction.
MedLine Citation:
PMID:  8035018     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The amount of human immunodeficiency virus type 1 (HIV-1) in various tissues was investigated by polymerase chain reaction (PCR) in 16 patients with end-stage HIV-1 infection and 7 patients with symptomatic but less advanced disease. During postmortem study of the 16 end-stage patients, HIV-1 DNA was found most often in lymph nodes and the spleen (both 100%), lung (93.8%), and colon (87.5%). Biopsied lymph nodes from the 7 symptomatic patients contained substantially higher copy numbers of HIV-1 RNA and DNA than did peripheral blood mononuclear cells (PBMC). Plasma viral RNA levels correlated significantly with the amount of HIV-1 RNA in PBMC (r2 = .86, P = .0025) but not with the level of viral RNA in lymph nodes in patients with symptomatic HIV-1 infection. These data suggest that although lymph nodes represent the main site for HIV-1 infection and replication, the level of circulating viral burden may not be solely determined by the magnitude of active HIV-1 replication in lymph nodes.
Authors:
S Sei; D E Kleiner; J B Kopp; R Chandra; P E Klotman; R Yarchoan; P A Pizzo; H Mitsuya
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of infectious diseases     Volume:  170     ISSN:  0022-1899     ISO Abbreviation:  J. Infect. Dis.     Publication Date:  1994 Aug 
Date Detail:
Created Date:  1994-08-18     Completed Date:  1994-08-18     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0413675     Medline TA:  J Infect Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  325-33     Citation Subset:  AIM; IM; X    
Affiliation:
Medicine Branch, National Cancer Institute, NIH, Bethesda, MD 20892.
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MeSH Terms
Descriptor/Qualifier:
Acquired Immunodeficiency Syndrome / microbiology*,  mortality
Adult
Central Nervous System / microbiology
Child
Child, Preschool
Colon / microbiology
DNA, Viral / analysis*
Female
Gene Expression Regulation, Viral
HIV-1 / genetics,  growth & development*,  physiology
Humans
Infant
Leukocytes, Mononuclear / microbiology
Lung / microbiology
Lymph Nodes / microbiology*
Lymphoid Tissue / microbiology
Male
Middle Aged
Polymerase Chain Reaction
RNA, Messenger / biosynthesis
RNA, Viral / analysis,  blood
Spleen / microbiology*
Thymus Gland / microbiology
Viremia / microbiology
Virus Replication
Chemical
Reg. No./Substance:
0/DNA, Viral; 0/RNA, Messenger; 0/RNA, Viral

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