Document Detail


Quantitative analysis of unconjugated and conjugated bile acids in duodenal fluid by densitometry after paper electrophoresis.
MedLine Citation:
PMID:  4698269     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new paper electrophoretic method for the separation of bile acids into five groups, (1) unconjugated, (2) glycine conjugates and (3) taurine conjugates, and (4) and (5) the respective monosulfates, is described. Rapid and accurate qualitative and quantitative estimations of each group are obtained by densitometry after internal standardization and phosphomolybdate color development. The technique can be done in the routine clinical laboratory and is useful for the detection of diseases affecting the enterohepatic circulation of bile acids.
Authors:
N Kaplowitz; N B Javitt
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of lipid research     Volume:  14     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1973 Mar 
Date Detail:
Created Date:  1973-06-19     Completed Date:  1973-06-19     Revised Date:  2007-03-21    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  224-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bile Acids and Salts / analysis*
Densitometry
Duodenum / secretion*
Electrophoresis, Paper
Gastrointestinal Diseases / metabolism
Glycocholic Acid / analysis
Sulfuric Acids / analysis
Taurocholic Acid / analysis
Chemical
Reg. No./Substance:
0/Bile Acids and Salts; 0/Sulfuric Acids; 475-31-0/Glycocholic Acid; 81-24-3/Taurocholic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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