Document Detail


Quantitative analysis of repair tissue biopsies following chondrocyte implantation.
MedLine Citation:
PMID:  12708659     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Outcome measures for cartilage repair techniques include clinical assessment of functional status, magnetic resonance imaging, mechanical indentation in situ and second-look biopsies, which are used for detailed ex vivo histological and immunohistochemical assessment. Biopsy analysis is considered an important outcome measure, despite being highly invasive, since it provides a visual record of the spatial organization of matrix proteins and cells. We propose that the value of second-look biopsies would be significantly enhanced if accurate quantification of cartilage matrix molecules could also be obtained. The goal of our work has been to develop a combined method for histological and biochemical analysis of a single biopsy. We have developed a method of cutting frozen sections of cartilage and recovering the uncut tissue for subsequent biochemical analysis. We have also developed a range of miniaturized assays that can be performed after cartilage digestion with trypsin. In this way we are now able to analyse biopsies with a wet weight as low as 5 mg using both histological and biochemical methods, so obtaining the maximum amount of information from the minimum volume of tissue. This new approach will allow a more accurate assessment of the quality of cartilage repair tissue than histological analysis alone.
Authors:
Anthony P Hollander; Sally C Dickinson; Trevor J Sims; Carlo Soranzo; Alessandra Pavesio
Related Documents :
17933559 - Effects of acl interference screws on articular cartilage volume and thickness measurem...
11044029 - Osteochondritis dissecans of the tibial plafond: imaging characteristics and a review o...
10693869 - 123i-antileukoproteinase scintigraphy reveals microscopic cartilage alterations in the ...
17966009 - High-resolution/high-contrast mri of human articular cartilage lesions.
16832129 - Patellofemoral full-thickness chondral defects treated with hyalograft-c: a clinical, a...
15568939 - Raman microspectroscopic mapping studies of human bronchial tissue.
21623319 - Guidance of magnetic resonance imaging and placement of skin-marker localization devices.
11886959 - Silicone breast implants: correlation between implant ruptures, magnetic resonance spec...
25022489 - Evaluation of the impact of organ-specific dose reduction on image quality in pediatric...
Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Novartis Foundation symposium     Volume:  249     ISSN:  1528-2511     ISO Abbreviation:  Novartis Found. Symp.     Publication Date:  2003  
Date Detail:
Created Date:  2003-04-23     Completed Date:  2003-06-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9807767     Medline TA:  Novartis Found Symp     Country:  England    
Other Details:
Languages:  eng     Pagination:  218-29; discussion 229-33, 234-8, 239-41     Citation Subset:  IM    
Affiliation:
University of Bristol Academic Rheumatology, Avon Orthopaedic Centre, Southmead Hospital, Bristol BS10 SNB, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Age Factors
Animals
Biocompatible Materials*
Biopsy
Cartilage, Articular / chemistry,  injuries,  pathology*,  surgery
Cattle
Cells, Cultured / chemistry,  transplantation
Chondrocytes / chemistry,  transplantation*
Collagen Type I / analysis
Collagen Type II / analysis
Endopeptidases / pharmacology
Enzyme-Linked Immunosorbent Assay
Follow-Up Studies
Frozen Sections
Glycosaminoglycans / analysis
Hyalin / chemistry
Hyaluronic Acid*
Hydroxyproline / analysis
Organ Size
Prostheses and Implants*
Rabbits
Second-Look Surgery
Surgical Mesh*
Tissue Engineering*
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/Collagen Type I; 0/Collagen Type II; 0/Glycosaminoglycans; 51-35-4/Hydroxyproline; 9004-61-9/Hyaluronic Acid; EC 3.4.-/Endopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Hyaluronan-based scaffolds (Hyalograft C) in the treatment of knee cartilage defects: preliminary cl...
Next Document:  Pathogen survival in swine manure environments and transmission of human enteric illness--a review.