Document Detail

Quantitative analysis of repair tissue biopsies following chondrocyte implantation.
MedLine Citation:
PMID:  12708659     Owner:  NLM     Status:  MEDLINE    
Outcome measures for cartilage repair techniques include clinical assessment of functional status, magnetic resonance imaging, mechanical indentation in situ and second-look biopsies, which are used for detailed ex vivo histological and immunohistochemical assessment. Biopsy analysis is considered an important outcome measure, despite being highly invasive, since it provides a visual record of the spatial organization of matrix proteins and cells. We propose that the value of second-look biopsies would be significantly enhanced if accurate quantification of cartilage matrix molecules could also be obtained. The goal of our work has been to develop a combined method for histological and biochemical analysis of a single biopsy. We have developed a method of cutting frozen sections of cartilage and recovering the uncut tissue for subsequent biochemical analysis. We have also developed a range of miniaturized assays that can be performed after cartilage digestion with trypsin. In this way we are now able to analyse biopsies with a wet weight as low as 5 mg using both histological and biochemical methods, so obtaining the maximum amount of information from the minimum volume of tissue. This new approach will allow a more accurate assessment of the quality of cartilage repair tissue than histological analysis alone.
Anthony P Hollander; Sally C Dickinson; Trevor J Sims; Carlo Soranzo; Alessandra Pavesio
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Novartis Foundation symposium     Volume:  249     ISSN:  1528-2511     ISO Abbreviation:  Novartis Found. Symp.     Publication Date:  2003  
Date Detail:
Created Date:  2003-04-23     Completed Date:  2003-06-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9807767     Medline TA:  Novartis Found Symp     Country:  England    
Other Details:
Languages:  eng     Pagination:  218-29; discussion 229-33, 234-8, 239-41     Citation Subset:  IM    
University of Bristol Academic Rheumatology, Avon Orthopaedic Centre, Southmead Hospital, Bristol BS10 SNB, UK.
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MeSH Terms
Age Factors
Biocompatible Materials*
Cartilage, Articular / chemistry,  injuries,  pathology*,  surgery
Cells, Cultured / chemistry,  transplantation
Chondrocytes / chemistry,  transplantation*
Collagen Type I / analysis
Collagen Type II / analysis
Endopeptidases / pharmacology
Enzyme-Linked Immunosorbent Assay
Follow-Up Studies
Frozen Sections
Glycosaminoglycans / analysis
Hyalin / chemistry
Hyaluronic Acid*
Hydroxyproline / analysis
Organ Size
Prostheses and Implants*
Second-Look Surgery
Surgical Mesh*
Tissue Engineering*
Reg. No./Substance:
0/Biocompatible Materials; 0/Collagen Type I; 0/Collagen Type II; 0/Glycosaminoglycans; 51-35-4/Hydroxyproline; 9004-61-9/Hyaluronic Acid; EC 3.4.-/Endopeptidases

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