Document Detail


Quantitative analysis of the relative transcript levels of ABC transporter Atr genes in Aspergillus nidulans by real-time reverse transcription-PCR assay.
MedLine Citation:
PMID:  11872487     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The development of assays for quantitative analysis of the relative transcript levels of ABC transporter genes by real-time reverse transcription-PCR (RT-PCR) might provide important information about multidrug resistance in filamentous fungi. Here, we evaluate the potential of real-time RT-PCR to quantify the relative transcript levels of ABC transporter Atr genes from Aspergillus nidulans. The AtrA to AtrD genes showed different and higher levels in the presence of structurally unrelated drugs, such as camptothecin, imazalil, itraconazole, hygromycin, and 4-nitroquinoline oxide. We also verified the relative transcript levels of the Atr genes in the A. nidulans imazalil-resistant mutants. These genes displayed a very complex pattern in different ima genetic backgrounds. The imaB mutant has higher basal transcript levels of AtrB and -D than those of the wild-type strain. The levels of these two genes are comparable when the imaB mutant is grown in the presence and absence of imazalil. The imaC, -D, and -H mutants have higher basal levels of AtrA than that of the wild type. The same behavior is observed for the relative transcript levels of AtrB in the imaG mutant background.
Authors:
Camile Pizeta Semighini; Mozart Marins; Maria Helena S Goldman; Gustavo Henrique Goldman
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  68     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-03-01     Completed Date:  2002-05-23     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1351-7     Citation Subset:  IM    
Affiliation:
Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café S/N, CEP 14040-903, Ribeirão Preto, São Paulo, Brazil.
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MeSH Terms
Descriptor/Qualifier:
ATP-Binding Cassette Transporters / genetics*,  metabolism
Antifungal Agents / pharmacology
Aspergillus nidulans / drug effects,  genetics,  metabolism*
Drug Resistance, Multiple, Fungal / genetics
Fungal Proteins / genetics,  metabolism
Mutation
Reverse Transcriptase Polymerase Chain Reaction*
Transcription, Genetic*
Chemical
Reg. No./Substance:
0/Antifungal Agents; 0/AtrC protein, Aspergillus nidulans; 0/Fungal Proteins
Comments/Corrections

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