| Quantitative analysis of phosphorothioate oligonucleotides in biological fluids using fast anion-exchange chromatography. | |
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MedLine Citation:
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PMID: 8376536 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phosphorothioate oligonucleotides are potentially useful as anti-viral drugs. Classical DNA extraction methods are not as effective on short single-stranded DNA as with longer double-stranced chains. The classical method of phenol-chloroform extraction followed by ethanol precipitation is difficult to quantify, thus monitoring of the pharmacological disposition of these compounds is subject to error. A method has been devised and validated for extraction and analysis of modified oligonucleotides from biological fluids such as urine and serum based on protein kinase digestion and phenol-chloroform extraction. Due to the high native ultraviolet absorbance of the oligomers, detection limits in the low ppb range were obtained without derivatization. |
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Authors:
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A J Bourque; A S Cohen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography Volume: 617 ISSN: 0021-9673 ISO Abbreviation: J. Chromatogr. Publication Date: 1993 Jul |
Date Detail:
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Created Date: 1993-10-18 Completed Date: 1993-10-18 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0427043 Medline TA: J Chromatogr Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 43-9 Citation Subset: IM |
Affiliation:
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Hybridon, Inc., Worcester, MA 01605. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Ion Exchange
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methods* Humans Oligonucleotides / analysis*, blood, urine Spectrophotometry, Ultraviolet Temperature Thionucleotides / analysis*, blood, urine |
| Chemical | |
Reg. No./Substance:
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0/Oligonucleotides; 0/Thionucleotides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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