Document Detail

Quantitative analysis of the modes of growth inhibition by weak organic acids in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  23001666     Owner:  NLM     Status:  MEDLINE    
Weak organic acids are naturally occurring compounds that are commercially used as preservatives in the food and beverage industries. They extend the shelf life of food products by inhibiting microbial growth. There are a number of theories that explain the antifungal properties of these weak acids, but the exact mechanism is still unknown. We set out to quantitatively determine the contributions of various mechanisms of antifungal activity of these weak acids, as well as the mechanisms that yeast uses to counteract their effects. We analyzed the effects of four weak organic acids differing in lipophilicity (sorbic, benzoic, propionic, and acetic acids) on growth and intracellular pH (pH(i)) in Saccharomyces cerevisiae. Although lipophilicity of the acids correlated with the rate of acidification of the cytosol, our data confirmed that not initial acidification, but rather the cell's ability to restore pH(i), was a determinant for growth inhibition. This pH(i) recovery in turn depended on the nature of the organic anion. We identified long-term acidification as the major cause of growth inhibition under acetic acid stress. Restoration of pH(i), and consequently growth rate, in the presence of this weak acid required the full activity of the plasma membrane ATPase Pma1p. Surprisingly, the proposed anion export pump Pdr12p was shown to play an important role in the ability of yeast cells to restore the pH(i) upon lipophilic (sorbic and benzoic) acid stress, probably through a charge interaction of anion and proton transport.
Azmat Ullah; Rick Orij; Stanley Brul; Gertien J Smits
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Publication Detail:
Type:  Journal Article     Date:  2012-09-21
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  78     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-09     Completed Date:  2013-04-15     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8377-87     Citation Subset:  IM    
Department of Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, Netherlands Institute for Systems Biology, University of Amsterdam, Amsterdam, the Netherlands.
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MeSH Terms
ATP-Binding Cassette Transporters / metabolism
Antifungal Agents / metabolism*
Carboxylic Acids / metabolism*
Cytosol / chemistry
Food Preservatives / metabolism*
Growth Inhibitors / metabolism*
Hydrogen-Ion Concentration
Proton-Translocating ATPases / metabolism
Saccharomyces cerevisiae / drug effects*,  growth & development*
Saccharomyces cerevisiae Proteins / metabolism
Reg. No./Substance:
0/Antifungal Agents; 0/Carboxylic Acids; 0/Food Preservatives; 0/Growth Inhibitors; 0/Pdr12 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; EC 3.6.1.-/PMA1 protein, S cerevisiae; EC ATPases

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