Document Detail

Quantitative analysis of gene expressions related to inflammation in canine spastic artery after subarachnoid hemorrhage.
MedLine Citation:
PMID:  11136939     Owner:  NLM     Status:  MEDLINE    
BACKGROUND AND PURPOSE: The possible role of inflammatory reaction of the cerebral artery in the pathogenesis of cerebral vasospasm has been noted in recent studies. We quantitatively measured the levels of expression of genes related to inflammation in the spastic artery in a canine double-hemorrhage model. METHODS: Twenty dogs were assigned to 4 groups: group D0, control; group D2, dogs killed 2 days after cisternal injection of blood; group D7, dogs given double cisternal injections of blood and killed 7 days after the first injection; and group D14. Angiography was performed twice: on the first day and before the animals were killed. Total RNA was extracted from the basilar artery. The expressions of interleukin (IL)-1alpha, IL-6, IL-8, IL-10, tumor necrosis factor-alpha, E-secretin, fibronectin, intercellular adhesion molecule (ICAM)-1, vascular cell adhesion molecule-1, transforming growth factor-ss, basic fibroblast growth factor, and collagen types I, III, and IV were examined with TaqMan real-time quantitative reverse transcription-polymerase chain reaction. RESULTS: Prolonged arterial narrowing peaking on 7 day was observed. There was a significant difference in vessel caliber between D0, D2, D7, and D14 groups (P:<0.0001). There were significant differences in mRNA expression in the basilar artery for IL-1alpha, IL-6, IL-8, ICAM-1, and collagen type I between D0, D2, D7, and D14 groups (P:=0.0079, 0. 0196, 0.0040, 0.0017, and <0.0001, respectively). The average level of mRNA was highest in D7 for IL-1alpha, IL-6, IL-8, and ICAM-1 (17-, 16-, 131-, and 1.7-fold compared with those of D0, respectively) and in D14 for collagen type I (10.9-fold). CONCLUSIONS: Increased expression of genes related to inflammation in the spastic artery suggests that inflammatory reaction of the cerebral artery is associated with sustained contraction.
Y Aihara; H Kasuya; H Onda; T Hori; J Takeda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Stroke; a journal of cerebral circulation     Volume:  32     ISSN:  1524-4628     ISO Abbreviation:  Stroke     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-01-12     Completed Date:  2001-01-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0235266     Medline TA:  Stroke     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  212-7     Citation Subset:  IM    
Department of Neurosurgery, Tokyo Women's Medical University, Tokyo, Japan.
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MeSH Terms
Basilar Artery / immunology,  metabolism*,  pathology
Cell Adhesion Molecules / genetics,  metabolism
Chemokines / genetics,  metabolism
Collagen / genetics,  metabolism
Cytokines / genetics,  metabolism
Disease Models, Animal
Extracellular Matrix / genetics,  metabolism
Fibroblast Growth Factor 2 / genetics,  metabolism
Gene Expression Profiling*
Inflammation / genetics*,  immunology,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Subarachnoid Hemorrhage / complications*
Transforming Growth Factor beta / genetics,  metabolism
Vasospasm, Intracranial / genetics*,  immunology,  metabolism
Reg. No./Substance:
0/Cell Adhesion Molecules; 0/Chemokines; 0/Cytokines; 0/Transforming Growth Factor beta; 103107-01-3/Fibroblast Growth Factor 2; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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