Document Detail

Quantitative analysis and engineering of fatty acid biosynthesis in E. coli.
MedLine Citation:
PMID:  20184964     Owner:  NLM     Status:  MEDLINE    
Fatty acids are central hydrocarbon intermediates in the biosynthesis of diesel from renewable sources. We have engineered an Escherichia coli cell line that produces 4.5 g/L/day total fatty acid in a fed-batch fermentation. However, further enhancement of fatty acid biosynthesis in this cell line proved unpredictable. To develop a more reliable engineering strategy, a cell-free system was developed that enabled direct, quantitative investigation of fatty acid biosynthesis and its regulation in E. coli. Using this system, the strong dependence of fatty acid synthesis on malonyl-CoA availability and several important phenomena in fatty acid synthesis were verified. Results from this cell-free system were confirmed via the generation and analysis of metabolically engineered strains of E. coli. Our quantitative findings highlight the enormous catalytic potential of the E. coli fatty acid biosynthetic pathway, and target specific steps for protein and metabolic engineering to enhance the catalytic conversion of glucose into biodiesel.
Tiangang Liu; Harmit Vora; Chaitan Khosla
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-02-23
Journal Detail:
Title:  Metabolic engineering     Volume:  12     ISSN:  1096-7184     ISO Abbreviation:  Metab. Eng.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-08-04     Completed Date:  2010-11-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9815657     Medline TA:  Metab Eng     Country:  Belgium    
Other Details:
Languages:  eng     Pagination:  378-86     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
Department of Chemistry, Stanford University, Stanford, CA 94305-5025, USA.
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MeSH Terms
Biosynthetic Pathways*
Escherichia coli / genetics,  metabolism*
Fatty Acids / biosynthesis*
Genetic Engineering
Glucose / metabolism
Malonyl Coenzyme A / metabolism
Reg. No./Substance:
0/Biofuels; 0/Fatty Acids; 50-99-7/Glucose; 524-14-1/Malonyl Coenzyme A

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