| Quantitative analysis of cell cycle phase durations and PC12 differentiation using fluorescent biosensors. | |
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MedLine Citation:
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PMID: 19270522 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell cycle analysis typically relies on fixed time-point measurements of cells in particular phases of the cell cycle. The cell cycle, however, is a dynamic process whose subtle shifts are lost by fixed time-point methods. Live-cell fluorescent biosensors and time-lapse microscopy allows the collection of temporal information about real time cell cycle progression and arrest. Using two genetically-encoded biosensors, we measured the precision of the G(1), S, G(2) and M cell cycle phase durations in different cell types and identified a bimodal G(1) phase duration in a fibroblast cell line that is not present in the other cell types. Using a cell line model for neuronal differentiation, we demonstrated that NGF-induced neurite extension occurs independently of NGF-induced cell cycle G(1) phase arrest. Thus, we have begun to use cell cycle fluorescent biosensors to examine the proliferation of cell populations at the resolution of individual cells and neuronal differentiation as a dynamic process of parallel cell cycle arrest and neurite outgrowth. |
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Authors:
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Angela T Hahn; Joshua T Jones; Tobias Meyer |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2009-04-02 |
Journal Detail:
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Title: Cell cycle (Georgetown, Tex.) Volume: 8 ISSN: 1551-4005 ISO Abbreviation: Cell Cycle Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-03-24 Completed Date: 2009-06-09 Revised Date: 2011-02-14 |
Medline Journal Info:
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Nlm Unique ID: 101137841 Medline TA: Cell Cycle Country: United States |
Other Details:
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Languages: eng Pagination: 1044-52 Citation Subset: IM |
Affiliation:
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Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biosensing Techniques / methods* Cell Cycle / physiology* Cell Division / physiology* Fibroblasts / cytology, physiology Fluorescence Hela Cells Humans Mice Nerve Growth Factor / metabolism Neurites / physiology* Neurogenesis / physiology* Neurons / cytology, physiology PC12 Cells Rats |
| Grant Support | |
ID/Acronym/Agency:
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R21 CA120732/CA/NCI NIH HHS; R21 CA120732-01/CA/NCI NIH HHS; R33 CA120732-02/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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9061-61-4/Nerve Growth Factor |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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