Document Detail


Quantitative analysis of cancer invasion in vitro: comparison of two new assays and of tumour sublines with different metastatic capacity.
MedLine Citation:
PMID:  3720058     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Various murine tumour sublines which differed considerably in their in vivo metastatic capacity were tested in vitro for their ability to invade normal tissue. For this purpose we developed two quantitative tests, a Boyden chamber endothelial cell invasion assay and a brain tissue microsphere invasion assay. The invasion of [75Se]methionine-prelabelled tumour cells into the normal tissues was followed by measuring the percentage of tumour-associated label in the brain microspheres or the endothelial monolayers after 12-48 h of co-cultivation. Clear and comparable differences existed in both assays between the amount of radiolabel found in the normal tissues after a co-cultivation with the different tumour lines. In three of the four tumour lines invasiveness correlated with metastatic capacity. The fourth line, a plastic adherent variant, was highly invasive but low metastatic. The ability of tumour cells to invade normal tissue, therefore, while necessary for the generation of metastases, is not in itself sufficient. Since both assays are independent of time-consuming histological sectioning and staining and allow a quantitative determination of invasive capacity of tumour cells grown as single cell suspensions they appear well suited for experimental manipulation and for screening of anti-invasive drugs.
Authors:
C A Waller; M Braun; V Schirrmacher
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical & experimental metastasis     Volume:  4     ISSN:  0262-0898     ISO Abbreviation:  Clin. Exp. Metastasis     Publication Date:    1986 Apr-Jun
Date Detail:
Created Date:  1986-07-29     Completed Date:  1986-07-29     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8409970     Medline TA:  Clin Exp Metastasis     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  73-89     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoradiography
Blood Vessels / pathology
Brain / pathology
Cell Adhesion
Cell Count
Cell Line
Cell Movement
Cells, Cultured
Endothelium / pathology
Lymphoma / chemically induced,  pathology*,  ultrastructure
Methylcholanthrene
Mice
Mice, Inbred DBA
Microspheres
Neoplasm Invasiveness / pathology*
Neoplasm Metastasis
Tumor Stem Cell Assay / methods
Chemical
Reg. No./Substance:
56-49-5/Methylcholanthrene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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