| Quantitative analysis of calcium (Ca2+) mobilization after stimulation with mitogens or anti-CD3 antibodies. Simultaneous fluo-3 and immunofluorescence flow cytometry. | |
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MedLine Citation:
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PMID: 1672879 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A method is described for flow microfluorometric analysis of calcium mobilization in immune cells. This method is based on dual-color analysis of heterogeneous cell populations using fluo-3 and phycoerythrin (PE)-conjugated monoclonal antibody (mAb). This technique allows the detection of fluo-3 fluorescence as a measure of an increase in cytoplasmic free calcium ([Ca2+]i) while simultaneously discriminating PE-mAb-labeled cells without using wavelength ratio imaging. This method provides a unique way to distinguish patterns of calcium mobilization within a heterogeneous cell population. |
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Authors:
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Y Sei; P K Arora |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of immunological methods Volume: 137 ISSN: 0022-1759 ISO Abbreviation: J. Immunol. Methods Publication Date: 1991 Mar |
Date Detail:
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Created Date: 1991-05-10 Completed Date: 1991-05-10 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 1305440 Medline TA: J Immunol Methods Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 237-44 Citation Subset: IM |
Affiliation:
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Laboratory of Neuroscience, NIDDK, National Institutes of Health, Bethesda, MD 20892. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aniline Compounds
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diagnostic use* Animals Antibodies, Monoclonal Antigens, CD / immunology* Antigens, CD3 Antigens, CD4 / immunology Antigens, CD8 Antigens, Differentiation, T-Lymphocyte / immunology* Antigens, Surface / immunology Antigens, Thy-1 Autoantibodies / pharmacology Calcium / analysis* Flow Cytometry Fluorescent Antibody Technique Lymphocyte Activation Mice Mice, Inbred C57BL Mitogens / pharmacology Phycoerythrin / diagnostic use Receptors, Antigen, T-Cell / immunology* T-Lymphocytes / immunology* Xanthenes / diagnostic use* |
| Chemical | |
Reg. No./Substance:
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0/Aniline Compounds; 0/Antibodies, Monoclonal; 0/Antigens, CD; 0/Antigens, CD3; 0/Antigens, CD4; 0/Antigens, CD8; 0/Antigens, Differentiation, T-Lymphocyte; 0/Antigens, Surface; 0/Antigens, Thy-1; 0/Autoantibodies; 0/Mitogens; 0/Receptors, Antigen, T-Cell; 0/Xanthenes; 11016-17-4/Phycoerythrin; 121714-13-4/Fluo-3; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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