Document Detail

Quantitative analysis of beta-phenylpyruvic acid by single ion monitoring. Evaluation of isomeric internal standards.
MedLine Citation:
PMID:  701420     Owner:  NLM     Status:  MEDLINE    
Quantitative single ion monitoring of beta-phenylpyruvic acid at high sensitivity is possible after derivatization first with omicron-phenylenediamine and then with a silylating reagent. The resulting o-trimethyl-silyl-quinoxalinol (O-TMS-Q) has previously been shown to be highly stable during storage and on chromatography. As an internal standard the isomeric omicron-methylphenylglyoxylic (omicron-toluylformic) acid is introduced. The mass spectra of both O-TMS-Q's are characterized by abundant [M]+. at m/e 308. The concept of "class specific metabolic profiling" is discussed in relation to quantitative gas chromatography--mass spectrometry detection of aliphatic and aromatic alpha-ketoacids.
U Langenbeck; A Mench-Hoinowski; I Rød-Urban
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography     Volume:  146     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1978 Sep 
Date Detail:
Created Date:  1978-12-29     Completed Date:  1978-12-29     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  213-9     Citation Subset:  IM    
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MeSH Terms
Chromatography, Gas
Mass Spectrometry
Phenylpyruvic Acids / analysis*
Reg. No./Substance:
0/Phenylpyruvic Acids

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