| Quantitative analysis of adhesion and biofilm formation on hydrophilic and hydrophobic surfaces of clinical isolates of Staphylococcus epidermidis. | |
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MedLine Citation:
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PMID: 15862449 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Staphylococcus epidermidis is now well established as a major nosocomial pathogen associated with infections of indwelling medical devices. The major virulence factor of these organisms is their ability to adhere to devices and form biofilms. However, it has not been established that adherence and biofilm formation are closely linked phenotypes for clinical isolates. In this study, the initial adhesion to different materials (acrylic and glass) of 9 clinical isolates of S. epidermidis, along with biofilm-positive and biofilm-negative control strains, was assayed using physico-chemical interactions to analyze the basis for bacterial adherence to the substratum. X-ray photo electron spectroscopy (XPS) analysis of the cell surface elemental composition was also performed in an attempt to find a relationship between chemical composition and adhesion capabilities. Biofilm formation on the two surfaces was evaluated by dry weight measurements. Human erythrocytes were used to evaluate the ability of S. epidermidis strains to cause hemagglutination, an indicator of the production of a poly-N-acetyl glucosamine cell surface polysaccharide also involved in biofilm formation. The clinical isolates exhibited different cell wall physico-chemical properties, resulting in differing abilities to adhere to surfaces. Adhesion to hydrophobic substrata for all strains occurred to a greater extent than that to hydrophilic surfaces. Bacterial cell hydrophobicity seemed to have little or no influence on adhesion. X-ray photoelectron spectroscopy analysis showed a high ratio of oxygen/carbon for all strains, which is a common characteristic of S. epidermidis species. No relevant relationship was found between XPS data and adhesion values. All strains forming biofilms were able to agglutinate erythrocytes. However, no direct relationship was found between the amount of biofilm formed and the initial adhesion extent. These results indicate that high levels of initial adherence do not necessarily lead to thick biofilm formation. These two aspects of the pathogenesis of medical device related-infection may need to be evaluated independently to ascertain the contribution of each to the virulence of S. epidermidis causing device-related infections. |
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Authors:
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Nuno Cerca; Gerald B Pier; Manuel Vilanova; Rosário Oliveira; Joana Azeredo |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. Date: 2005-02-19 |
Journal Detail:
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Title: Research in microbiology Volume: 156 ISSN: 0923-2508 ISO Abbreviation: Res. Microbiol. Publication Date: 2005 May |
Date Detail:
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Created Date: 2005-05-02 Completed Date: 2006-03-14 Revised Date: 2013-05-13 |
Medline Journal Info:
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Nlm Unique ID: 8907468 Medline TA: Res Microbiol Country: France |
Other Details:
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Languages: eng Pagination: 506-14 Citation Subset: IM |
Affiliation:
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Centro de Engenharia Biológica, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acrylates Bacterial Adhesion* Biofilms / growth & development* Glass Humans Hydrophobic and Hydrophilic Interactions Staphylococcus epidermidis / chemistry, isolation & purification, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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AI 46706/AI/NIAID NIH HHS; R01 AI046706/AI/NIAID NIH HHS; R01 AI046706-05/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Acrylates |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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