Document Detail

Quantitative analysis of GBV-C RNA in liver and serum by strand-specific reverse transcription-polymerase chain reaction.
MedLine Citation:
PMID:  9833907     Owner:  NLM     Status:  MEDLINE    
BACKGROUND/AIMS: Although GB virus C (GBV-C) is frequently detectable in patients with liver diseases, it is not known whether it actually replicates in the liver. Therefore we have quantitatively examined the strand-specific RNA of this virus. METHODS: Fourteen patients (two GBV-C RNA only seropositive, seven both GBV-C RNA and HCV RNA seropositive, and five HCV RNA only seropositive) were examined. Extracted RNAs from sera and liver specimens of these patients were serially diluted and strand-specific RNAs of GBV-C and HCV were quantitatively measured using strand-specific primers by reverse transcription-polymerase chain reaction. RESULTS: Positive-strand GBV-C RNA in serum was detected in all nine GBV-C RNA seropositive cases, and negative-strand RNA was detected in three, uncertain in three, and undetected in three. Positive-strand GBV-C RNA in the liver was detected in seven and undetected in two, while negative-strand RNA in the liver was undetected in eight and uncertain in one. On the other hand, positive-strand HCV RNA was detected in serum from all 12 HCV RNA seropositive patients and negative-strand HCV RNA was detected in one, uncertain in seven, and undetected in four. Positive-strand HCV RNA was detected in the liver from all 12 HCV RNA seropositive patients, and the presence of negative-strand HCV RNA in the liver was confirmed in 10 and uncertain in the remaining two. CONCLUSION: GBV-C is considered to be far less hepatotropic than HCV, and it is suggested that GBV-C might not be replicating in the liver.
T Kanda; O Yokosuka; M Tagawa; S Kawai; F Imazeki; H Saisho
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of hepatology     Volume:  29     ISSN:  0168-8278     ISO Abbreviation:  J. Hepatol.     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1999-02-10     Completed Date:  1999-02-10     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8503886     Medline TA:  J Hepatol     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  707-14     Citation Subset:  IM    
First Department of Medicine, Chiba University School of Medicine, Japan.
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MeSH Terms
Blood / virology
DNA Primers
Flaviviridae / genetics,  isolation & purification*
Hepatitis, Viral, Human / blood,  virology*
Liver / virology
Middle Aged
Polymerase Chain Reaction
RNA, Viral / analysis*
Reg. No./Substance:
0/DNA Primers; 0/RNA, Viral

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