Document Detail

Quantitative amino acid analysis using a Beckman system gold HPLC 126AA analyzer.
MedLine Citation:
PMID:  11580921     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The Beckman 6300/7300 analyzer, which was widely used for amino acid (AA) analysis, is no longer commercially available. METHODS: To set up an affordable AA analysis program, a Beckman system gold HPLC 126AA analyzer and Pickering Laboratories reagents were used. Two quantitative AA analysis programs were developed. One was an 18-min short program quantitating seven AAs from plasma and dried blood spots (DBS) specimens using Lithium eluents Li-365 and Li-375 at 70 degrees C column temperature. The short program could be used for diagnosis and follow-up dietary management for phenylketonuria (PKU), maple syrup urine disease (MSUD), tyrosinemia and homocystinuria patients. The second program was a 118-min long AA screening panel quantitating 40 AAs using Lithium eluents Li-275, Li-365 and Li-375 at 32, 48 and 72 degrees C column temperatures from plasma and urine specimens. RESULTS: The values obtained from DBS specimens were in good agreement with certified results from the Centers for Disease Control and Prevention. The values obtained from plasma and urine samples were in good correlation with those obtained from Beckman 6300 analyzer (0.9076 < or = r < or = 0.999). CONCLUSIONS: Amino acid quantitation from physiological samples using a Beckman 126AA Analyzer and Pickering Laboratories reagents was useful for clinical diagnosis and monitoring of aminoacidopathies.
Y Qu; R H Slocum; J Fu; W E Rasmussen; H D Rector; J B Miller; J G Coldwell
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  312     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-10-02     Completed Date:  2002-02-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  153-62     Citation Subset:  IM    
H.A. Chapman Institute of Medical Genetics, 5300 East Skelly Drive, Tulsa, OK 74135, USA.
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MeSH Terms
Amino Acids / blood*,  urine*
Blood Specimen Collection / methods
Chromatography, High Pressure Liquid / methods*
Homocystinuria / diagnosis,  metabolism
Image Processing, Computer-Assisted
Maple Syrup Urine Disease / diagnosis,  metabolism
Phenylketonurias / diagnosis,  metabolism
Tyrosinemias / diagnosis,  metabolism
Reg. No./Substance:
0/Amino Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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