Document Detail

Quantitative Vibrational Imaging by Hyperspectral Stimulated Raman Scattering Microscopy and Multivariate Curve Resolution Analysis.
MedLine Citation:
PMID:  23198914     Owner:  NLM     Status:  Publisher    
Spectroscopic imaging has been an increasingly critical approach for unveiling specific molecules in biological environments. Towards this goal, we demonstrate hyperspectral stimulated Raman loss (SRL) imaging by intra-pulse spectral scanning through a femtosecond pulse shaper. The hyperspectral stack of SRL images is further analyzed by a multivariate curve resolution (MCR) method to reconstruct quantitative concentration images for each individual component and retrieve the corresponding vibrational Raman spectra. Using these methods, we demonstrate quantitative mapping of dimethyl sulfoxide concentration in aqueous solutions and in fat tissue. Moreover, MCR is performed on SRL images of breast cancer cells to generate maps of principal chemical components along with their respective vibrational spectra. These results show the great capability and potential of hyperspectral SRL microscopy for quantitative imaging of complicated biomolecule mixtures through resolving overlapped Raman bands.
Delong Zhang; Ping Wang; Mikhail N Sliphenko; Dor Ben Amotz; Andrew M Weiner; Ji-Xin Cheng
Related Documents :
23262684 - Three dimensional hilo-based structured illumination for a digital scanned laser sheet ...
24402894 - Fabrication and performance of a miniaturized 64-element high-frequency endoscopic phas...
25064554 - Targeted multimodal imaging modalities.
23411434 - Medical imaging: just what the doctor (and the researcher) ordered: new applications fo...
18684694 - On the notion of 'software independence' in voting systems.
11339564 - Experience with a dynamic inexpensive video-conferencing system for frozen section tele...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-3
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Alcohol and young people: What the legislation says about access and secondary supply.
Next Document:  Metabolic profiles characterizing different phenotypes of polycystic ovary syndrome: plasma metabolo...