Document Detail

A quantitative real-time PCR assay for quantification of viable Listeria monocytogenes cells after bacteriocin injury in food-first insights.
MedLine Citation:
PMID:  20419373     Owner:  NLM     Status:  In-Process    
Quantitative real-time PCR may be a rapid and automated procedure for detection of bacterial pathogens from food samples. Nevertheless, when testing the effects of antimicrobials on the viability of bacterial pathogens in foods, we found that DNA from dead cells interfered greatly in the detection of viable Listeria monocytogenes after treatment with the broad-spectrum bacteriocin enterocin AS-48. To overcome this problem, a quantitative real-time PCR (qRT-PCR) assay based on bacterial mRNA was adapted to quantify viable L. monocytogenes in food after bacteriocin treatments. The procedure allowed a better and faster estimation of viable cells compared to PALCAM viable cell counts when the threshold level was 2 log units/g of food, while PALCAM viable count allowed detection of one log unit/g. This procedure may be useful to verify the efficacy of bacteriocins against L. monocytogenes in foods.
Antonio Cobo Molinos; Hikmate Abriouel; Nabil Ben Omar; Magdalena Martinez-Canamero; Antonio Gálvez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-24
Journal Detail:
Title:  Current microbiology     Volume:  61     ISSN:  1432-0991     ISO Abbreviation:  Curr. Microbiol.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7808448     Medline TA:  Curr Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  515-9     Citation Subset:  IM    
Health Science Department, University of Jaén, Jaén, Spain.
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