Document Detail


A quantitative rabbit model of vaccinia keratitis.
MedLine Citation:
PMID:  20375331     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: The goal of this study was to use multiple quantitative disease measures to evaluate the effect of various viral inocula on the development of vaccinia keratitis in rabbits.
METHODS: Trephined eyes of female rabbits were infected with 10(4), 10(5), 10(6), or 10(7) plaque-forming units (pfu) of the Dryvax strain of the vaccinia virus and scored daily for disease for 14 days according to a modification of the MacDonald-Shadduck scoring system. Ocular viral titers and vaccinia-specific antibody titers were determined by plaque assay and ELISA, respectively.
RESULTS: The amount of virus used for infection affected the severity of disease, with 10(4) pfu eliciting milder keratitis after delayed onset compared with higher amounts of virus. At inocula above 10(5) pfu the course and severity of corneal disease was not significantly different. The time to reach peak titers was delayed in the 10(4) group but peak titers were similar in all groups. Severe conjunctival chemosis interfered with scoring in animals infected with 10(6) or 10(7) pfu. Virus-specific antibody titers were similar in all groups at day 14. Body weights decreased less than 10% in all groups.
CONCLUSIONS: The course of vaccinia keratitis in rabbits paralleled that in humans. A viral inoculum of 10(5) pfu/eye was determined to be optimal for use in further studies of vaccinia keratitis.
Authors:
Sharon Altmann; Andrew Emanuel; Megan Toomey; Kim McIntyre; Jill Covert; Richard Redd Dubielzig; Gary Leatherberry; Christopher J Murphy; Shantha Kodihalli; Curtis R Brandt
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.     Date:  2010-04-07
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  51     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-31     Completed Date:  2010-10-04     Revised Date:  2014-09-08    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4531-40     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Viral / blood
Body Weight
Cercopithecus aethiops
Disease Models, Animal*
Female
Keratitis / physiopathology,  virology*
Lip / virology
Rabbits*
Severity of Illness Index
Skin Ulcer / virology
Smallpox Vaccine / adverse effects*,  immunology
Vaccinia / immunology,  physiopathology*
Vaccinia virus / growth & development*,  immunology
Vero Cells
Grant Support
ID/Acronym/Agency:
AGRDTD 05/09/07//PHS HHS; P30 EY016665/EY/NEI NIH HHS; P30 EY016665/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Viral; 0/Smallpox Vaccine
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