Document Detail

Quantitative PCR approach to SNP detection and linkage mapping in Caenorhabditis elegans.
MedLine Citation:
PMID:  17140115     Owner:  NLM     Status:  MEDLINE    
I report a method for single nucleotide polymorphism (SNP) detection and linkage mapping in Caenorhabditis elegans using automated oligonucleotide design and fluorescence-based quantitative PCR detection. Nine hundred twenty-three oligonucleotide pairs were designed to produce small products of <150 bp for efficient amplification in a PCR, with one oligonucleotide of each pair overlapping a SNP site at the 3'-most nucleotide. A subset of the pairs were tested, and efficient allelic discrimination was obtained for SNPs between N2, the canonical laboratory strain, and CB4856, a strain isolated from Hawaii commonly used for mapping studies. Linkage mapping is demonstrated using the unc-119 locus of C. elegans. This quantitative PCR method provides an inexpensive, uniform, and automatable detection alternative for genetic mapping strategies in C. elegans or other organisms.
Christopher A Shelton
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  BioTechniques     Volume:  41     ISSN:  0736-6205     ISO Abbreviation:  BioTechniques     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-12-04     Completed Date:  2006-12-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8306785     Medline TA:  Biotechniques     Country:  United States    
Other Details:
Languages:  eng     Pagination:  583-8     Citation Subset:  IM    
Department of Pathway Genomics, GlaxoSmithKline, Collegeville, PA 19426, USA.
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MeSH Terms
Caenorhabditis elegans / genetics*
Chromosome Mapping*
Polymerase Chain Reaction / methods*
Polymorphism, Single Nucleotide*

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