| Quantitative magnetic resonance imaging assessment of matrix development in cell-seeded natural urinary bladder smooth muscle tissue-engineered constructs. | |
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MedLine Citation:
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PMID: 19772478 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The approach of cell-seeded natural scaffolds holds great promise for tissue engineering complicated soft-tissue organs such as the urinary bladder and heart. However, relatively little is known about cell-natural scaffold interactions or their influence on magnetic resonance imaging (MRI) characterization, which is valuable for noninvasive monitoring. Ideally, MRI should provide information on tissue biochemistry in addition to structure and function. In this study, quantitative MRI was performed on control and smooth muscle cell-seeded natural bladder matrices at different time points up to 7 days postseeding. Measurements of MR relaxation times (T1 and T2) and diffusion coefficient (D) showed an overall change that was incompatible with cell presence. Multicomponent T2 provided greater specificity, revealing time-course changes in the short T2 fraction that were consistent with biochemically determined matrix degradation from collagenase released from seeded cells. These matrix alterations are noted for the first time, and their relatively early occurrence may be unique to soft-tissue matrices compared with synthetic materials. More importantly, they are not evident on histology but are revealed on quantitative MRI. We conclude that quantitative MRI may provide specific information on cell-matrix interaction and is a promising noninvasive approach to understand and monitor cell-seeded natural scaffold-based regeneration. |
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Authors:
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Hai-Ling Margaret Cheng; Syed S Islam; Yasir Loai; Roula Antoon; Marine Beaumont; Walid A Farhat |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Tissue engineering. Part C, Methods Volume: 16 ISSN: 1937-3392 ISO Abbreviation: Tissue Eng Part C Methods Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-26 Completed Date: 2010-10-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101466663 Medline TA: Tissue Eng Part C Methods Country: United States |
Other Details:
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Languages: eng Pagination: 643-51 Citation Subset: IM |
Affiliation:
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Department of Medical Biophysics, University of Toronto, Toronto, Canada. hai-ling.cheng@sickkids.ca |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Assay Collagen Type I / metabolism Collagenases / metabolism Diffusion Extracellular Matrix / metabolism* Fluorescent Antibody Technique Magnetic Resonance Imaging* Muscle, Smooth / physiology* Myocytes, Smooth Muscle / cytology*, metabolism Sus scrofa Time Factors Tissue Engineering / methods* Tissue Scaffolds / chemistry* Urinary Bladder / physiology* |
| Grant Support | |
ID/Acronym/Agency:
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//Canadian Institutes of Health Research |
| Chemical | |
Reg. No./Substance:
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0/Collagen Type I; EC 3.4.24.-/Collagenases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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