| Quantitative loss of heterozygosity analysis for urothelial carcinoma detection and prognosis. | |
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MedLine Citation:
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PMID: 20206968 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: To evaluate loss of heterozygosity (LOH) using microsatellite polymorphism analysis as a diagnostic and prognostic marker at the time of transurethral resection and as a follow-up marker preceding cystoscopic evidence of recurrence compared with cytology. METHODS: A total of 127 urothelial carcinoma (UC) patients were included. Tumors were staged and graded according to the International Union Against Cancer-tumor, node, metastases system and to the 2004 World Health Organization classification. LOH urinalysis was performed using 8 markers and marker-specific LOH thresholds. Thirty control samples, obtained from healthy volunteers, were used to determine the positive cut-off for each marker. RESULTS: LOH was significantly more sensitive than cytology in low-grade (64.8% vs 38.5%, P <.001) and low-stage UC (68.6% vs 45.5%, P <.001). The cumulative sensitivity of cytology and LOH reached 74.7% (P <.001) for low-grade and 80.2% (P <.001) for low-stage tumors. Both urinary LOH at TP53 and chromosome 9p markers were associated with an increased risk of recurrence (relative risk = 1.73 [1.30-2.31], P = .0002) and occurred more frequently in the initial urine samples of patients who later relapsed from primary tumors (36.4% vs 0.0%, P <.05 and 57.6% vs 15.8%, P = .0001). Among 32 relapse patients, LOH was positive alongside cystoscopy in 25 of 32 cases and tested positive before cystoscopy detected recurrence in a further 5 of 25 cases. CONCLUSIONS: UC diagnosis and monitoring would greatly benefit from supplementing conventional cytology with LOH urinalysis, using a panel of 8 microsatellite markers with specific threshold levels. Given the limitations of both cystoscopy and cytology, novel molecular markers are needed for detection and follow-up of UC. |
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Authors:
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Delphine Collin-Chavagnac; Christophe Marçais; Stephane Billon; Françoise Descotes; Eric Piaton; Myriam Decaussin; Claire Rodriguez-Lafrasse; Alain Ruffion |
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Publication Detail:
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Type: Journal Article Date: 2010-03-05 |
Journal Detail:
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Title: Urology Volume: 76 ISSN: 1527-9995 ISO Abbreviation: Urology Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-10 Completed Date: 2010-09-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0366151 Medline TA: Urology Country: United States |
Other Details:
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Languages: eng Pagination: 515.e1-7 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Service de Biochimie et Biologie Moléculaire Sud, Centre Hospitalier Universitaire Lyon Sud, Chemin du Grand Revoyet, Pierre Bénite, Hospices Civils de Lyon, France. delphine.collin_chavagnac@bbox.fr |
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| MeSH Terms | |
Descriptor/Qualifier:
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Aged Aged, 80 and over Carcinoma, Transitional Cell / genetics* Female Humans Loss of Heterozygosity* Male Middle Aged Prognosis Prospective Studies Sensitivity and Specificity Urinary Bladder Neoplasms / genetics* |
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