Document Detail

Quantitative fundus autofluorescence in healthy eyes.
MedLine Citation:
PMID:  23860757     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Fundus autofluorescence was quantified (qAF) in subjects with healthy retinae using a standardized approach. The objective was to establish normative data and identify factors that influence the accumulation of RPE lipofuscin and/or modulate the observed AF signal in fundus images.
METHODS: AF images were acquired from 277 healthy subjects (age range: 5-60 years) by employing a Spectralis confocal scanning laser ophthalmoscope (cSLO; 488-nm excitation; 30°) equipped with an internal fluorescent reference. For each image, mean gray level was calculated as the average of eight preset regions, and was calibrated to the reference, zero-laser light, magnification, and optical media density from normative data on lens transmission spectra. Relationships between qAF and age, sex, race/ethnicity, eye color, refraction/axial length, and smoking status were evaluated as was measurement repeatability and the qAF spatial distribution.
RESULTS: qAF levels exhibited a significant increase with age. qAF increased with increasing eccentricity up to 10° to 15° from the fovea and was highest superotemporally. qAF values were significantly greater in females, and, compared with Hispanics, qAF was significantly higher in whites and lower in blacks and Asians. No associations with axial length and smoking were observed. For two operators, between-session repeatability was ± 9% and ± 12%. Agreement between the operators was ± 13%.
CONCLUSIONS: Normative qAF data are a reference tool essential to the interpretation of qAF measurements in ocular disease.
Jonathan P Greenberg; Tobias Duncker; Russell L Woods; R Theodore Smith; Janet R Sparrow; François C Delori
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Validation Studies     Date:  2013-08-21
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  54     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2013  
Date Detail:
Created Date:  2013-08-22     Completed Date:  2013-10-31     Revised Date:  2014-02-04    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5684-93     Citation Subset:  IM    
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MeSH Terms
Child, Preschool
Continental Population Groups
Fundus Oculi
Lipofuscin / metabolism
Melanins / metabolism
Middle Aged
Ophthalmoscopy / methods*,  standards*
Optical Imaging / instrumentation,  methods*,  standards*
Reference Values
Retina / cytology,  metabolism*
Retinal Pigment Epithelium / cytology,  metabolism*
Young Adult
Grant Support
R01 EY015520/EY/NEI NIH HHS; R01 EY015520/EY/NEI NIH HHS; R24 EY019861/EY/NEI NIH HHS; R24 EY019861/EY/NEI NIH HHS
Reg. No./Substance:
0/Lipofuscin; 0/Melanins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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