| Quantitative Evaluation of Bioorthogonal Chemistries for Surface Functionalization of Nanoparticles. | |
| | |
MedLine Citation:
|
PMID: 23153257 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
We present here a highly efficient and chemoselective liposome functionalization method based on oxime bond formation between a hydroxylamine and an aldehyde modified lipid component. We have conducted a systematic and quantitative comparison of this new approach with other state-of-the-art conjugation reactions in the field. Targeted liposomes that recognize over-expressed receptors or antigens on diseased cells have great potential in therapeutic and diagnostic applications. However, chemical modifications of nanoparticle surfaces by post-functionalization approaches are less effective than in solution and often not high yielding. In addition, the conjugation efficiency is often challenging to characterize and therefore not addressed in many reports. We here present an investigation of PEGylated liposomes functionalized with a neuroendocrine tumor targeting peptide (TATE); synthesized with variety of functionalities that have been used for surface conjugation of nanoparticles. The reaction kinetics and overall yield was quantified by HPLC. Reactions were conducted in solution as well as by post-functionalization of liposomes in order to study the effects of steric hindrance and possible affinity between the peptide and liposome surface. These studies demonstrate the importance of choosing the correct chemistry in order to obtain a quantitative surface functionalization of liposomes. |
| | |
Authors:
|
Lise N Feldborg; Rasmus I Jølck; Thomas L Andresen |
Related Documents
:
|
15120297 - True molar surface energy and alignment of surface molecules. 21345587 - Synthesis of surface imprinted nanospheres for selective removal of uranium from simula... 23589687 - Facile one-step coating approach to magnetic submicron particles with poly(ethylene gly... 15377517 - Stabilization of internal charges in a protein: water penetration or conformational cha... 20735677 - Clues to the location of the spawning area and larval migration characteristics of angu... 3411467 - Nuclear magnetic resonance study of an ethyl cellulose sustained-release delivery syste... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-11-16 |
Journal Detail:
|
Title: Bioconjugate chemistry Volume: - ISSN: 1520-4812 ISO Abbreviation: Bioconjug. Chem. Publication Date: 2012 Nov |
Date Detail:
|
Created Date: 2012-11-16 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9010319 Medline TA: Bioconjug Chem Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effect of relative humidity on the migration of benzophenone from paperboard into the food simulant ...
Next Document: Helminth-infected patients with malaria: a low profile transmission hub?