Document Detail


Quantitative Evaluation of Bioorthogonal Chemistries for Surface Functionalization of Nanoparticles.
MedLine Citation:
PMID:  23153257     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We present here a highly efficient and chemoselective liposome functionalization method based on oxime bond formation between a hydroxylamine and an aldehyde modified lipid component. We have conducted a systematic and quantitative comparison of this new approach with other state-of-the-art conjugation reactions in the field. Targeted liposomes that recognize over-expressed receptors or antigens on diseased cells have great potential in therapeutic and diagnostic applications. However, chemical modifications of nanoparticle surfaces by post-functionalization approaches are less effective than in solution and often not high yielding. In addition, the conjugation efficiency is often challenging to characterize and therefore not addressed in many reports. We here present an investigation of PEGylated liposomes functionalized with a neuroendocrine tumor targeting peptide (TATE); synthesized with variety of functionalities that have been used for surface conjugation of nanoparticles. The reaction kinetics and overall yield was quantified by HPLC. Reactions were conducted in solution as well as by post-functionalization of liposomes in order to study the effects of steric hindrance and possible affinity between the peptide and liposome surface. These studies demonstrate the importance of choosing the correct chemistry in order to obtain a quantitative surface functionalization of liposomes.
Authors:
Lise N Feldborg; Rasmus I Jølck; Thomas L Andresen
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-16
Journal Detail:
Title:  Bioconjugate chemistry     Volume:  -     ISSN:  1520-4812     ISO Abbreviation:  Bioconjug. Chem.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9010319     Medline TA:  Bioconjug Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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