Document Detail


Quantitative Determination of Vinblastine in Tissue Cultures of Catharanthus roseus by Radioimmunoassay.
MedLine Citation:
PMID:  17262413     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A radioimmunoassay has been developed for the quantitative determination of small amounts of vinblastine in tissue cultures of CATHARANTHUS ROSEUS (L.) G. Don. Antibody was obtained by the immunization of rabbits against a conjugate of vinblastine with bovine serum albumin. The antibody had a high affinity (Ka = 1.2 x 10 (9)l/mol) and specificity for vinblastine. The usable range of standard curve for assay was 0.5-10 ng/ml. Crude alkaloid extracts of tissue cultures could be assayed and many samples could be processed in one time. It was found that the vinblastine contents of multiple shoot cultures were lower than that of intact plants but much higher than that of callus cultures.
Authors:
K Hirata; M Kobayashi; K Miyamoto; T Hoshi; M Okazaki; Y Miura
Related Documents :
18726273 - Fine structure and assembly in vitro of nuclear lamina in plant cells.
18838783 - Isolation and photoisomerization of a new anthraquinone from hairy root cultures of ses...
17942163 - Growth inhibition of cultured marine phytoplankton by toxic algal-derived polyunsaturat...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Planta medica     Volume:  55     ISSN:  0032-0943     ISO Abbreviation:  Planta Med.     Publication Date:  1989 Jun 
Date Detail:
Created Date:  2007-01-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0066751     Medline TA:  Planta Med     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  262-4     Citation Subset:  -    
Affiliation:
Department of Biochemical Engineering, Faculty of Pharmaceutical Sciences, Osaka University, 1-6, Yamadaoka, Suita, 565, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Volatile garlic odor components: gas phases and adsorbed exhaled air analysed by headspace gas chrom...
Next Document:  Investigation of the Anti-Snake Venom Activity of Schumanniophyton magnificum.