Document Detail

Quantitative Determination of Vinblastine in Tissue Cultures of Catharanthus roseus by Radioimmunoassay.
MedLine Citation:
PMID:  17262413     Owner:  NLM     Status:  In-Data-Review    
A radioimmunoassay has been developed for the quantitative determination of small amounts of vinblastine in tissue cultures of CATHARANTHUS ROSEUS (L.) G. Don. Antibody was obtained by the immunization of rabbits against a conjugate of vinblastine with bovine serum albumin. The antibody had a high affinity (Ka = 1.2 x 10 (9)l/mol) and specificity for vinblastine. The usable range of standard curve for assay was 0.5-10 ng/ml. Crude alkaloid extracts of tissue cultures could be assayed and many samples could be processed in one time. It was found that the vinblastine contents of multiple shoot cultures were lower than that of intact plants but much higher than that of callus cultures.
K Hirata; M Kobayashi; K Miyamoto; T Hoshi; M Okazaki; Y Miura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Planta medica     Volume:  55     ISSN:  0032-0943     ISO Abbreviation:  Planta Med.     Publication Date:  1989 Jun 
Date Detail:
Created Date:  2007-01-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0066751     Medline TA:  Planta Med     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  262-4     Citation Subset:  -    
Department of Biochemical Engineering, Faculty of Pharmaceutical Sciences, Osaka University, 1-6, Yamadaoka, Suita, 565, Japan.
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