| Quantitative Detection of Aspergillus spp. by Real-Time Nucleic Acid Sequence-Based Amplification. | |
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MedLine Citation:
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PMID: 23296887 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Rapid and quantitative detection of Aspergillus from clinical samples may facilitate an early diagnosis of invasive pulmonary aspergillosis (IPA). As nucleic acid-based detection is a viable option, we demonstrate that Aspergillus burdens can be rapidly and accurately detected by a novel real-time nucleic acid assay other than qPCR by using the combination of nucleic acid sequence-based amplification (NASBA) and the molecular beacon (MB) technology. Here, we detail a real-time NASBA assay to determine quantitative Aspergillus burdens in lungs and bronchoalveolar lavage (BAL) fluids of rats with experimental IPA. |
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Authors:
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Yanan Zhao; David S Perlin |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 968 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2013-01-08 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 83-92 Citation Subset: IM |
Affiliation:
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Public Health Research Institute Center, UMDNJ-NJMS, Newark, NJ, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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