Document Detail


Quantitative CT imaging of the spatio-temporal distribution patterns of vasa vasorum in aortas of apoE-/-/LDL-/- double knockout mice.
MedLine Citation:
PMID:  20692662     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To investigate the distribution of vasa vasorum (VV) relative to advanced atherosclerotic lesions (calcified, fibrotic or hemorrhaged) along the aortic wall of apoE-/-/LDL-/- mice at the age of 25 and 80 weeks using high-resolution nano-CT.
METHODS: Aortas from male apoE-/-/LDL-/- mice at the age of 25 weeks (n=4) and 80 weeks (n=7) were infused in situ with contrast agent and harvested for scanning with nano-CT. The spatial distribution of vasa vasorum [number and area/cross-section (mm2)] was compared to aortic luminal cross-sectional area and plaque cross-sectional area in the ascending aorta, aortic arch and descending aorta. Results were complemented with co-localized histology.
RESULTS: The number and total luminal cross-sectional area of VV showed a significant decrease in the ascending aorta and aortic arch from 25 to 80 weeks but not in the descending aorta. The number and cross-sectional area of VV showed significant local differences depending on whether it was near a fibrotic, and hemorrhaged or calcified plaque in animals at the age of 80 weeks. Area of VV progressively increased along the aorta from least in the ascending aorta<aortic arch<descending aorta in animals at the age of 80 weeks and is inverse in animals aged 25 weeks.
CONCLUSION: Atherosclerotic lesion type is correlated to the number and cross-sectional area of VV in different aortic segments in apoE-/-/LDL-/- mice. The chronological development of VV along the aorta proceeds distally from the ascending aorta and aortic arch.
Authors:
M Kampschulte; A Brinkmann; P Stieger; D G Sedding; C Dierkes; R M Bohle; G Krombach; Erik L Ritman; A C Langheinrich
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-07-17
Journal Detail:
Title:  Atherosclerosis     Volume:  212     ISSN:  1879-1484     ISO Abbreviation:  Atherosclerosis     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-11     Completed Date:  2011-03-02     Revised Date:  2013-12-06    
Medline Journal Info:
Nlm Unique ID:  0242543     Medline TA:  Atherosclerosis     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  444-50     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aorta / pathology*
Apolipoproteins E / genetics*
Atherosclerosis / pathology
Imaging, Three-Dimensional
Lipoproteins, LDL / genetics*
Male
Mice
Mice, Knockout
Mice, Transgenic
Neovascularization, Pathologic
Plaque, Atherosclerotic / pathology*
Time Factors
Tomography, X-Ray Computed / methods*
Vasa Vasorum / pathology*
Grant Support
ID/Acronym/Agency:
EB000305/EB/NIBIB NIH HHS; HL65342/HL/NHLBI NIH HHS; R01 EB000305/EB/NIBIB NIH HHS; R01 HL065342/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Apolipoproteins E; 0/Lipoproteins, LDL

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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