Document Detail

Quantitative Assessment of the Relative Antineoplastic Potential of the n-butanolic Leaf Extract of Annona Muricata Linn. in Normal and Immortalized Human Cell Lines.
MedLine Citation:
PMID:  22524847     Owner:  NLM     Status:  In-Data-Review    
Natural products have been the target for cancer therapy for several years but there is still a dearth of information on potent compounds that may protect normal cells and selectively destroy cancerous cells. The present study was aimed to evaluate the cytotoxic potential of n-butanolic leaf extract of Annona muricata L. on WRL-68 (normal human hepatic cells), MDA-MB-435S (human breast carcinoma cells) and HaCaT (human immortalized keratinocyte cells) lines by XTT assay. Prior to cytotoxicity testing, the extract was subjected to phytochemical screening for detecting the presence of compounds with therapeutic potential. Their relative antioxidant properties were evaluated using the reducing power and DPPH* radical scavenging assay. Since most of the observed chemo-preventive potential invariably correlated with the amount of total phenolics present in the extract, their levels were quantified and identified by HPLC analysis. Correlation studies indicated a strong and significant (P<0.05) positive correlation of phenolic compounds with free radical scavenging potential. The results revealed that the extract was moderately cytotoxic to normal cells with a mean IC50 value of 52.4μg when compared with those obtained for cancerous cells (IC50 values of 29.2μg for MDA-MB-435S and 30.1μg for HaCaT respectively). The study confirms the presence of therapeutically active antineoplastic compounds in the n-butanolic leaf extract of Annona muricata. Isolation of the active metabolites from the extract is in prospect.
V Cijo George; Dr Naveen Kumar; V Rajkumar; Pk Suresh; R Ashok Kumar
Related Documents :
21367017 - 31 development and apoptosis in bovine cloned embryos reconstructed with oocytes collec...
8180777 - Immunogold analysis of antioxidant enzymes in human renal cell carcinoma.
2721807 - Clear-cell syringoma. immunohistochemistry and electron microscopy study.
9218707 - Detachment of glycolytic enzymes from cytoskeleton of melanoma cells induced by calmodu...
20878707 - Neuroprotective effect of honokiol and magnolol, compounds from magnolia officinalis, o...
15109857 - Colonization and maintenance of murine embryonic stem cells on poly(alpha-hydroxy esters).
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Asian Pacific journal of cancer prevention : APJCP     Volume:  13     ISSN:  1513-7368     ISO Abbreviation:  Asian Pac. J. Cancer Prev.     Publication Date:  2012  
Date Detail:
Created Date:  2012-04-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101130625     Medline TA:  Asian Pac J Cancer Prev     Country:  Thailand    
Other Details:
Languages:  eng     Pagination:  699-704     Citation Subset:  IM    
School of Biosciences and Technology, VIT University, Vellore, India E-mail
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Clinicopathologic profile of breast cancer patients in pakistan: ten years data of a local cancer ho...
Next Document:  Factors Associated With Smoking, Quit Attempts and Attitudes towards Total Smoking Bans at Universit...