| Quantitative Analysis of Topoisomerase II Alpha and Evaluation of Its Effects on Cell Proliferation and Apoptosis in Glioblastoma Cancer Stem Cells. | |
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MedLine Citation:
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PMID: 22569122 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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DNA topoisomerase II alpha (Topo-IIα) is an essential nuclear enzyme with its up-regulation demonstrated in different tumors. This study is to evaluate the expression of Topo-IIα in glioblastoma cancer stem cells (CSCs) and its effects on cell proliferation and apoptosis. We observed the expression differences of Topo-IIα gene transcript in CSCs and non-CSCs in glioblastoma cell line U87 by quantitative real-time PCR and western blot; we silenced Topo-IIα expression in U87 CSCs using Topo-IIα-specific siRNA to investigate the role of Topo-IIα in cell proliferation, cell cycle, and apoptosis. We found higher expression of Topo-IIα in CSCs than in non-CSCs. After using Topo-IIα-specific siRNA to silence the expression of Topo-IIα gene, we observed decreasing cell proliferation, cell cycle arrest, and induction of apoptosis in CSCs, which suggest that Topo-IIα contributes to both cell proliferation and/or cell survival. Topo-IIα may be a novel marker of cell proliferation and/or survival factor in glioblastoma CSCs. It may be a potential target that will open up new opportunities to develop therapeutic strategies for gliomas. |
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Authors:
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Yang Hong; Meng Sang; Chao Shang; Yi-Xue Xue; Yun-Hui Liu |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-5-5 |
Journal Detail:
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Title: Neuroscience letters Volume: - ISSN: 1872-7972 ISO Abbreviation: - Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-5-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7600130 Medline TA: Neurosci Lett Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012. Published by Elsevier Ireland Ltd. |
Affiliation:
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Department of Neurosurgery, Shengjing Hospital, China Medical University, Shenyang, 110004, China; Department of Neurobiology, College of Basic Medicine, China Medical University, Shenyang, 110001, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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