Document Detail

Quantitative Analysis of Molecular Transport across Liposomal Bilayer by J-mediated 13C Overhauser Dynamic Nuclear Polarization.
MedLine Citation:
PMID:  23072518     Owner:  NLM     Status:  Publisher    
We introduce a new NMR technique to dramatically enhance the solution-state 13C NMR sensitivity and contrast at 0.35 T and at room temperature by actively transferring the spin polarization from Overhauser Dynamic Nuclear Polarization (ODNP)-enhanced 1H to 13C nuclei through scalar (J) coupling, a method that we term J-mediated 13C ODNP. We demonstrate the capability of this technique by quantifying the permeability of glycine across negatively charged liposomal bilayers composed of dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylglycerol (DPPG). The permeability coefficient of glycine across this DPPC/DPPG bilayer is measured to be (1.8±0.1)×10-11m/s, in agreement with the literature value. We further observed that the presence of 20 mol% cholesterol within the DPPC/DPPG lipid membrane significantly retards the permeability of glycine by a factor of 4. These findings demonstrate that the high sensitivity and contrast of J-mediated 13C ODNP affords to measure the permeation kinetics of small hydrophilic molecules across lipid bilayers, a quantity that is difficult to accurately measure with existing techniques.
Chi-Yuan Cheng; Olga J G M Goor; Songi Han
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-16
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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