| Quantitative Analysis of Molecular Transport across Liposomal Bilayer by J-mediated 13C Overhauser Dynamic Nuclear Polarization. | |
| | |
MedLine Citation:
|
PMID: 23072518 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
We introduce a new NMR technique to dramatically enhance the solution-state 13C NMR sensitivity and contrast at 0.35 T and at room temperature by actively transferring the spin polarization from Overhauser Dynamic Nuclear Polarization (ODNP)-enhanced 1H to 13C nuclei through scalar (J) coupling, a method that we term J-mediated 13C ODNP. We demonstrate the capability of this technique by quantifying the permeability of glycine across negatively charged liposomal bilayers composed of dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylglycerol (DPPG). The permeability coefficient of glycine across this DPPC/DPPG bilayer is measured to be (1.8±0.1)×10-11m/s, in agreement with the literature value. We further observed that the presence of 20 mol% cholesterol within the DPPC/DPPG lipid membrane significantly retards the permeability of glycine by a factor of 4. These findings demonstrate that the high sensitivity and contrast of J-mediated 13C ODNP affords to measure the permeation kinetics of small hydrophilic molecules across lipid bilayers, a quantity that is difficult to accurately measure with existing techniques. |
| | |
Authors:
|
Chi-Yuan Cheng; Olga J G M Goor; Songi Han |
Related Documents
:
|
16907398 - Constituents of the quasiparticle spectrum along the nodal direction of high-tc cuprates. 23418558 - Ion concentration-dependent ion conduction mechanism of a voltage-sensitive potassium c... 23215508 - Origin of the structural phase transition in li_{7}la_{3}zr_{2}o_{12}. 15697938 - Surface and bulk spin ordering of antiferromagnetic materials: nio(111). 11134418 - Flower stalk segments of arabidopsis thaliana ecotype columbia lack the capacity to gro... 15697298 - Direct electrochemical interaction between a modified gold electrode and a bacterial me... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-10-16 |
Journal Detail:
|
Title: Analytical chemistry Volume: - ISSN: 1520-6882 ISO Abbreviation: Anal. Chem. Publication Date: 2012 Oct |
Date Detail:
|
Created Date: 2012-10-17 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Randomized controlled trial of a teleconference fatigue management plus physical activity interventi...
Next Document: Dronedarone-Associated Acute Renal Failure: Evidence Coming from the Italian Spontaneous ADR Reporti...