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Quantitative Analysis of Fetal DNA In Maternal Plasma In Gestational Diabetes Mellitus, Iron Deficiency Anemia And Gestational Hypertension Pregnancies.
MedLine Citation:
PMID:  23339569     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abstract Objective: to quantify circulating fetal DNA (fDNA) levels in the second and third trimesters of normal healthy pregnant individuals and pregnant women with the following clinical conditions: gestational diabetes mellitus (GDM), iron deficiency anemia and gestational hypertension (GHT). Methods: The SRY gene which is located on Y chromosome was used as a unique fetal marker. The fDNA was extracted from maternal plasma and the SRY gene concentrations were measured by quantitative real-time polymerase chain reaction (PCR) amplification using TaqMan dual labeled probe system. Results: No significant differences were observed in the mean fDNA concentration between normal and GDM pregnancy samples (P > 0.05) and also between normal and anemic pregnancy samples (P > 0.05) in both trimesters, but significant differences were observed between the third trimester normal and GHT pregnancy samples (P = 0.001). GDM and iron deficiency anemia does not affect levels of fDNA in maternal plasma while GHT significantly elevates the levels of fDNA in maternal plasma. Conclusions: Increased amount of circulating fDNA in maternal plasma could be used for early identification of adverse pregnancies. GDM and anemia does not affect levels of fDNA in maternal plasma while GHT significantly elevates the levels of fDNA in maternal plasma. Hence, the elevated fDNA values could be used as potential screening marker in pregnancies complicated with GHT but not with GDM and iron deficiency anemia.
Authors:
Mansour Zamanpoor; Rozita Rosli; Mohd Nazri Yazid; Zaheed Husian; Norshariza Nordin; Karuppiah Thilakavathy
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-22
Journal Detail:
Title:  The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians     Volume:  -     ISSN:  1476-4954     ISO Abbreviation:  J. Matern. Fetal. Neonatal. Med.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101136916     Medline TA:  J Matern Fetal Neonatal Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Genetic Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang UPM, Selangor, Malaysia.
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