Document Detail


Quantitative Analysis and Characterization of Self-Assembled DNA on a Silver Surface.
MedLine Citation:
PMID:  23025347     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Self-assembled monolayers of DNA on a silver surface were prepared and characterized by polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS), fluorescence imaging, and (32)P radioactive labeling. The buffer concentration of the DNA solution and the surface roughness of the silver substrate were found to affect the surface coverage of DNA and its hybridization. At low buffer concentrations, surface coverage and hybridization were greatly reduced. Ethidium bromide intercalated into the adsorbed dsDNA clearly indicates the presence of dsDNA.
Authors:
Karuppannan Senthil Kumar; Ron Naaman
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-3
Journal Detail:
Title:  Langmuir : the ACS journal of surfaces and colloids     Volume:  -     ISSN:  1520-5827     ISO Abbreviation:  Langmuir     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882736     Medline TA:  Langmuir     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Chemical Physics, The Weizmann Institute of Science , Rehovot 76100, Israel.
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