Document Detail


Quantitative 19F NMR study of trifluorothymidine metabolism in rat brain.
MedLine Citation:
PMID:  10516619     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Metabolism of trifluorothymidine (TFT) and its transport across the blood-brain barrier (BBB) has been measured quantitatively in rats by fluorine-19 nuclear magnetic resonance spectroscopy ((19)F NMR). It is demonstrated that TFT crosses the BBB in micromolar quantities and is metabolized in brain tissue primarily to its free base trifluoromethyluracil (TFMU) by the enzyme thymidine phosphorylase (TP). It is further proposed that the rate of TFMU production can be used as a measure of cerebral TP. The glycols of both TFMU, and to a lesser degree TFT, are generated via an oxidative route. In contrast, the major pathway for hepatic metabolism of this compound is through reduction of the nitrogen base moiety and generation of 5-6-dihydro species followed by ring degradation. Thus, in addition to TFMU as well as the dihydroxy (glycol)-, and the dihydro-species of both TFT and TFMU, alpha-trifluoromethyl-beta-ureidopropionic acid (F(3)MUPA) and alpha-trifluoromethyl-beta-alanine (F(3)MBA) were detected in liver extracts. The total metabolite levels in liver were 2-5 times higher than in the brain. Low levels of fluoride ion were detected in all the extracts from brain and liver, as well as blood and urine. This study characterizes TFT as a potential chemotherapeutic agent for use against brain tumors.
Authors:
R Pouremad; K D Bahk; Y J Shen; R H Knop; A M Wyrwicz
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  NMR in biomedicine     Volume:  12     ISSN:  0952-3480     ISO Abbreviation:  NMR Biomed     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-12-22     Completed Date:  1999-12-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8915233     Medline TA:  NMR Biomed     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  373-80     Citation Subset:  IM    
Copyright Information:
Copyright 1999 John Wiley & Sons, Ltd.
Affiliation:
Department of Neurobiology and Physiology and the Lurie Comprehensive Cancer Center, Northwestern University and Northwestern University Medical School, Evanston, IL 60201, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antimetabolites / metabolism*,  pharmacokinetics
Biological Transport
Blood-Brain Barrier
Brain / enzymology,  metabolism*
Liver / enzymology,  metabolism
Magnetic Resonance Spectroscopy
Pyrimidines / metabolism
Rats
Rats, Sprague-Dawley
Thymidine Phosphorylase / metabolism
Trifluridine / metabolism*,  pharmacokinetics
Grant Support
ID/Acronym/Agency:
GM53175/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Antimetabolites; 0/Pyrimidines; 54-20-6/trifluoromethyluracil; 70-00-8/Trifluridine; EC 2.4.2.4/Thymidine Phosphorylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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