Document Detail


Quantitation of plasma (13) C-galactose and (13) C-glucose during exercise by liquid chromatography/isotope ratio mass spectrometry.
MedLine Citation:
PMID:  21818809     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The utilisation of carbohydrate sources under exercise conditions is of considerable importance in performance sports. Incorporation of optimal profiles of macronutrients can improve endurance performance in athletes. However, gaining an understanding of the metabolic partitioning under sustained exercise can be problematical and isotope labelling approaches can help quantify substrate utilisation. The utilisation of oral galactose was investigated using (13) C-galactose and measurement of plasma galactose and glucose enrichment by liquid chromatography/isotope ratio mass spectrometry (LC/IRMS). As little as 100 μL plasma could readily be analysed with only minimal sample processing. Fucose was used as a chemical and isotopic internal standard for the quantitation of plasma galactose and glucose concentrations, and isotopic enrichment. The close elution of galactose and glucose required a correction routine to be implemented to allow the measurement, and correction, of plasma glucose δ(13) C, even in the presence of very highly enriched galactose. A Bland-Altman plot of glucose concentration measured by LC/IRMS against glucose measured by an enzymatic method showed good agreement between the methods. Data from seven trained cyclists, undergoing galactose supplementation before exercise, demonstrate that galactose is converted into glucose and is available for subsequent energy metabolism. Copyright © 2011 John Wiley & Sons, Ltd.
Authors:
Douglas J Morrison; John P O'Hara; Roderick F G J King; Tom Preston
Related Documents :
16996909 - Resting energy expenditure and metabolic changes after lung volume reduction surgery fo...
7665419 - Energy expenditure during antiorthostatic bed rest (simulated microgravity).
21957449 - How reproductive ecology contributes to the spread of a globally invasive fish.
21795999 - Blood flow restriction exercise in sprinters and endurance runners.
9771879 - Balancing energy expenditure and body weight.
9506799 - Evaluation of a commercial accelerometer (tritrac-r3 d) to measure energy expenditure d...
20350669 - The effect of spinal manipulation on the efficacy of a rehabilitation protocol for pati...
12900689 - Effect of repeated caffeine ingestion on repeated exhaustive exercise endurance.
12695989 - Phenotypic flexibility of body composition in relation to migratory state, age, and sex...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Rapid communications in mass spectrometry : RCM     Volume:  25     ISSN:  1097-0231     ISO Abbreviation:  Rapid Commun. Mass Spectrom.     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-08-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8802365     Medline TA:  Rapid Commun Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  2484-8     Citation Subset:  -    
Copyright Information:
Copyright © 2011 John Wiley & Sons, Ltd.
Affiliation:
Stable Isotope Biochemistry Laboratory, SUERC, Rankine Avenue, East Kilbride, G75 0QF, UK. douglas.morrison@glasgow.ac.uk.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Protein identification by accurate mass matrix-assisted laser desorption/ionization imaging of trypt...
Next Document:  Diagnostic cyclisation reactions which follow phosphate transfer to carboxylate anion centres for en...