Document Detail

Quantitation of olanzapine in tablets by HPLC, CZE, derivative spectrometry and linear voltammetry.
MedLine Citation:
PMID:  11095298     Owner:  NLM     Status:  MEDLINE    
Four analytical methods have been developed for the quality control of pharmaceutical formulations containing the novel antipsychotic drug, olanzapine: high performance liquid chromatography (HPLC), capillary zone electrophoresis (CZE), derivative spectrometry and linear voltammetry. All methods require only a simple extraction procedure of olanzapine from the tablets before analysis. HPLC with ultraviolet detection at 260 nm is carried out with a C8 column and a mobile phase constituted of acetonitrile and aqueous tetramethylammonium perchlorate. CZE is performed in an uncoated capillary with phosphate buffer, pH 3.0, as the background electrolyte, with UV detection at 214 nm. Spectrophotometry uses the derivative of the spectrum at 298 nm. In linear voltammetric method (LSV) the current intensity of the oxidation wave at +495 mV is measured. All methods gave similar results in terms of precision and accuracy. For HPLC and CZE, repeatability and intermediate precision, expressed by the RSD was better than 1.8%. The accuracy, resulting from recovery experiments, was between 99.9 and 101.1%. Spectrometry and voltammetry gave slightly higher RSD values (up to 2.9%) and a larger variation of the accuracy (the recovery was between 97.8 and 102.6%). However, the requirements for quantitative analysis are fulfilled for all methods.
M A Raggi; G Casamenti; R Mandrioli; G Izzo; E Kenndler
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  23     ISSN:  0731-7085     ISO Abbreviation:  J Pharm Biomed Anal     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2001-03-08     Completed Date:  2001-05-31     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  973-81     Citation Subset:  IM    
Department of Pharmaceutical Sciences, University of Bologna, Italy.
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MeSH Terms
Antipsychotic Agents / analysis*
Chromatography, High Pressure Liquid / methods*
Electrophoresis, Capillary / methods*
Pirenzepine / analogs & derivatives*,  analysis
Reproducibility of Results
Spectrophotometry / methods*
Reg. No./Substance:
0/Antipsychotic Agents; 0/Tablets; 12794-10-4/Benzodiazepines; 132539-06-1/olanzapine; 28797-61-7/Pirenzepine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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