| Quantitation of nanogram amounts of nucleic acids in the presence of proteins by the ethidium bromide staining technique. | |
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MedLine Citation:
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PMID: 1708186 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Modification of the method for determining low amounts of RNA and DNA is proposed. It consists in nucleic acid staining in solution with EtBr (1 microgram/ml) followed by photography of 10 microliters drops on a UV-transparent plate under UV illumination. Densitometric measurements of the Polaroid negatives were used to construct standard concentration curves in the range of 1-16 micrograms/ml of DNA or RNA. This permitted to determine nucleic acid in amounts as little as 10 micrograms. The measurements were not influenced by the presence of proteins such as bovine serum albumin, DNase, RNase or proteinase K, thus the method proposed may be useful in determining the nucleic acid content of very small samples or of scarce biological material. |
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Authors:
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B Olszańska; A Borgul |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Acta biochimica Polonica Volume: 37 ISSN: 0001-527X ISO Abbreviation: Acta Biochim. Pol. Publication Date: 1990 |
Date Detail:
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Created Date: 1991-05-17 Completed Date: 1991-05-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 14520300R Medline TA: Acta Biochim Pol Country: POLAND |
Other Details:
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Languages: eng Pagination: 59-63 Citation Subset: IM |
Affiliation:
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Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals DNA / analysis* Densitometry / methods Embryo, Nonmammalian Ethidium Female Fluorescence Mice Microchemistry Oocytes / chemistry Quail RNA / analysis* Staining and Labeling |
| Chemical | |
Reg. No./Substance:
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3546-21-2/Ethidium; 63231-63-0/RNA; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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