| Quantitation and immunohistochemical localization of cathepsins E and D in rat tissues and blood cells. | |
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MedLine Citation:
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PMID: 2655714 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The distribution of cathepsins E and D in various rat tissues and blood cells was determined by immunoprecipitation and by immunohistochemistry with discriminative antibodies specific for each enzyme. While cathepsin D was detected in all of the tissues and blood cells tested (except for erythrocytes), cathepsin E had a relatively limited distribution. The cathepsin E content was highest in the stomach and was succeeded in the following order by the urinary bladder, thymus, spleen, cervical lymph node and bone marrow. Significant amounts of cathepsin E were also found in the colon, rectum, jejunum, skin, lung, kidney and submandibular gland. The other tissues tested had little or no detectable cathepsin E content. Of the blood cells tested, lymphocytes and peritoneal neutrophils contained high levels of cathepsin E. Erythrocytes had cathepsin E only as aspartic proteinases. When the subcellular localization of cathepsin E in the neutrophils was investigated by fractionation of the postnuclear supernatants, the enzyme behaved as a soluble cytosolic enzyme. In contrast, cathepsin D was mainly associated with the granular fraction. The immunohistochemical localization of cathepsins E and D was clearly different in the stomach, large intestines, kidney and urinary bladder, but was similar in the lymph node and spleen. The tissue-fixed macrophages, which were notable in the skeletal and cardiac muscle tissues, submucosal layers of the gastrointestinal tracts, salivary gland, lung and trachea, also exhibited similar intense immunoreactivities demonstrative of both cathepsins E and D. |
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Authors:
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H Sakai; T Saku; Y Kato; K Yamamoto |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 991 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1989 May |
Date Detail:
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Created Date: 1989-07-11 Completed Date: 1989-07-11 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 367-75 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, Nagasaki University School of Dentistry, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acid Phosphatase
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metabolism Alkaline Phosphatase / metabolism Animals Antibodies Cathepsin D / blood, metabolism* Cathepsin E Cathepsins / blood, metabolism* Digestive System / enzymology Immunoenzyme Techniques Immunohistochemistry Kinetics L-Lactate Dehydrogenase / metabolism Lymphoid Tissue / enzymology Neutrophils / enzymology Organ Specificity Rats Subcellular Fractions / enzymology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 3.1.3.1/Alkaline Phosphatase; EC 3.1.3.2/Acid Phosphatase; EC 3.4.-/Cathepsins; EC 3.4.23.34/Cathepsin E; EC 3.4.23.5/Cathepsin D |
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