Document Detail


Quantitation of human protein S in the plasma of normal and warfarin-treated individuals by radioimmunoassay.
MedLine Citation:
PMID:  6241357     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Protein S was purified from human plasma and used to raise monospecific antibodies. A double antibody equilibrium radioimmunoassay was constructed and met all the requirements for a sensitive, accurate and specific determination of Protein S. The sensitivity of the assay was between 8-250 ng of Protein S/ml and the coefficient of variation was 2-6% within assays and 13-19% between assays. Complement C4b-binding protein or Protein C did not effect the measurement of Protein S. Complete competition with parallel slopes of inhibition was seen among purified Protein S and plasma from normal and warfarin-treated individuals indicating immunochemical identity and validating the measurement of Protein S in plasma. The concentration of Protein S in normal plasma (n=24) was 23.4 +/- 4.42. micrograms/ml and in plasma from warfarin-treated individuals (n=24) was 12.6 +/- 3.92 micrograms/ml. Thus, a common feature of individuals undergoing warfarin therapy is the reduction of approximately 50% in the concentration of each of the vitamin K-dependent proteins.
Authors:
D S Fair; D J Revak
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Thrombosis research     Volume:  36     ISSN:  0049-3848     ISO Abbreviation:  Thromb. Res.     Publication Date:  1984 Dec 
Date Detail:
Created Date:  1985-04-03     Completed Date:  1985-04-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0326377     Medline TA:  Thromb Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  527-35     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / immunology
Glycoproteins / blood*,  immunology
Humans
Immunochemistry
Protein S
Radioimmunoassay / methods*
Warfarin / therapeutic use*
Grant Support
ID/Acronym/Agency:
HL-16411/HL/NHLBI NIH HHS; HL-28672/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Glycoproteins; 0/Protein S; 81-81-2/Warfarin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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