| Quantitation of the epsilon-(gamma-glutamyl)lysine cross-link using a high-speed amino acid analyzer without purification of the dipeptide. Application to enzymatic digested mixtures of keratin and the membranous fraction of human stratum corneum. | |
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MedLine Citation:
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PMID: 6192134 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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epsilon-(gamma-Glutamyl)lysine in an enzymically digested mixture of keratin and the membranous fraction of human stratum corneum was directly quantitated using a high-speed amino acid analyzer without purification of the dipeptide. The analytical conditions were improved so that epsilon-(gamma-glutamyl)lysine clearly separated from other amino acids and eluted directly after tyrosine. The enzymatically digested mixtures were filtered through an Ultra Free membrane and deammoniated before analysis. By our present method, keratin and the membranous fraction of human stratum corneum were analyzed and 5.8 and 43.5 nmol/mg of epsilon-(gamma-glutamyl)lysine, respectively, were detected. |
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Authors:
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K Murayama; T Sugawara; E Hiraga; H Ogawa |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography Volume: 274 ISSN: 0021-9673 ISO Abbreviation: J. Chromatogr. Publication Date: 1983 May |
Date Detail:
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Created Date: 1983-09-09 Completed Date: 1983-09-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0427043 Medline TA: J Chromatogr Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 63-73 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Autoanalysis / methods Chromatography, Ion Exchange Dipeptides / isolation & purification* Enzymes Humans Keratins / analysis* Methods Skin / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Dipeptides; 0/Enzymes; 17105-15-6/epsilon-(gamma-glutamyl)-lysine; 68238-35-7/Keratins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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